Recombinant Human CD10 protein (ab155720)
- Datasheet
- References
- Protocols
Overview
-
Product nameRecombinant Human CD10 protein
See all CD10 proteins and peptides -
Protein lengthProtein fragment
Description
-
NatureRecombinant
-
SourceHEK 293 cells
-
Amino Acid Sequence
-
Accession
-
SpeciesHuman
-
SequenceYDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETS SRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDS RGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLI NLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMIS VARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYN KMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEY LTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGT TSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVF IQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELN YKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRN QIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDG DLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIAD NGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPE YAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW
-
Molecular weight107 kDa
-
Amino acids52 to 750
-
Specifications
Our Abpromise guarantee covers the use of ab155720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Endotoxin level< 1.000 Eu/µg
-
Purity>95% by SDS-PAGE .
-
FormLyophilised
-
Additional notesab155720 migrates at 120-130 kDa in reduced SDS-PAGE due to glycosylation.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.00
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris -
ReconstitutionIt is recommended to reconstitute ab155720 in sterile PBS to a final concentration of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. Avoid vigorous shaking or vortexing.
General Info
-
Alternative names
- Atriopeptidase
- CALLA
- CD10
see all -
FunctionThermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
-
Sequence similaritiesBelongs to the peptidase M13 family.
-
Cellular localizationCell membrane.
- Information by UniProt
Images
-
SDS-PAGE - Recombinant Human CD10 protein (ab155720)SDS-PAGE of reduced ab155720 stained overnight with Coomassie Blue.
Datasheets and documents
References
ab155720 has not yet been referenced specifically in any publications.
