Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
      P08473
    • Species
      Human
    • Sequence
      YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETS SRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDS RGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLI NLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMIS VARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYN KMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEY LTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGT TSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVF IQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELN YKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRN QIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDG DLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIAD NGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPE YAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW
    • Molecular weight
      107 kDa
    • Amino acids
      52 to 750

Specifications

Our Abpromise guarantee covers the use of ab155720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Additional notes
    ab155720 migrates at 120-130 kDa in reduced SDS-PAGE due to glycosylation.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.00
    Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris

  • Reconstitution
    It is recommended to reconstitute ab155720 in sterile PBS to a final concentration of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. Avoid vigorous shaking or vortexing.

General Info

  • Alternative names
    • Atriopeptidase
    • CALLA
    • CD10
    • CD10 antigen
    • Common acute lymphocytic leukemia antigen
    • DKFZp686O16152
    • EC 3.4.24.11
    • Enkephalinase
    • EPN
    • Membrane metallo endopeptidase
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase)
    • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)
    • Membrane metallo endopeptidase variant 1
    • Membrane metallo endopeptidase variant 2
    • Membrane metalloendopeptidase
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase
    • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10
    • Membrane metalloendopeptidase variant 1
    • Membrane metalloendopeptidase variant 2
    • MGC126681
    • MGC126707
    • MME
    • NEP
    • NEP_HUMAN
    • Neprilysin
    • neprilysin-390
    • neprilysin-411
    • Neutral endopeptidase
    • Neutral endopeptidase 24.11
    • Neutral endopeptidase, membrane-associated
    • SFE
    • Skin fibroblast elastase
    see all
  • Function
    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • Sequence similarities
    Belongs to the peptidase M13 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

Images

  • SDS-PAGE - CD10 protein (Fc Chimera) (ab155720)
    SDS-PAGE - CD10 protein (Fc Chimera) (ab155720)
    SDS-PAGE of reduced ab155720 stained overnight with Coomassie Blue.

References

ab155720 has not yet been referenced specifically in any publications.

Publishing research using ab155720? Please let us know so that we can cite the reference in this datasheet.

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