Recombinant Human CD10 protein (ab155720)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: Fc tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human CD10 protein
See all CD10 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETS SRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDS RGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLI NLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMIS VARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYN KMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEY LTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGT TSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVF IQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELN YKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRN QIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDG DLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIAD NGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPE YAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW -
Predicted molecular weight
107 kDa including tags -
Amino acids
52 to 750 -
Tags
Fc tag N-Terminus -
Additional sequence information
This protein carries a human IgG1 Fc tag at the N-terminus.
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Specifications
Our Abpromise guarantee covers the use of ab155720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: Tris, Glycine, L-Arginine, Sodium chloride -
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- Atriopeptidase
- CALLA
- CD10
see all -
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human CD10 protein (ab155720)
ab155720 on SDS-PAGE under reducing conditions. The gel was stained overnight with Coomassie Blue. The protein migrates as 106-120 kDa under reducing conditions due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab155720 has not yet been referenced specifically in any publications.
