Recombinant human CD10 protein (ab157051)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CD10 protein
See all CD10 proteins and peptides -
Biological activity
1140 pmol/min/µg (9 ng NEP were used to cleave 10µM Mca-RPPGFSAFK(Dnp)-OH (Fluorogenic Substrate) at 37°C in 50 mM TRIS, pH 9.0).
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Purity
> 95 % SDS-PAGE.
ab157051 has been purified in the glycosylated soluble form. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSS RYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSR GGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLIN LFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISV ARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNK MTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYL TKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTT SETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFI QTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNY KEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQ IVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGD LVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADN GGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEY AVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW -
Predicted molecular weight
85 kDa including tags -
Amino acids
53 to 750 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab157051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Stable on ice for at least 1h. Do not freeze in dilute form. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituents: 0.61% Tris, 20% Glycerol (glycerin, glycerine), 1.75% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Atriopeptidase
- CALLA
- CD10
see all -
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
Sequence similarities
Belongs to the peptidase M13 family. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant human CD10 protein (ab157051)SDS-PAGE Analysis of 1.0 μg of purified ab157051.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab157051 has been referenced in 1 publication.
- Yu S et al. A CD10-OGP Membrane Peptolytic Signaling Axis in Fibroblasts Regulates Lipid Metabolism of Cancer Stem Cells via SCD1. Adv Sci (Weinh) 8:e2101848 (2021). PubMed: 34363355
