Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Description
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Product name
Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active)
See all CD134 / OX40L receptor proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA Immobilized Recombinant human OX40L/TNFSF4 protein (His tag) (ab221412) at 5 μg/mL (100 μL/well) can bind ab221340 with a linear range of 2-25 ng/mL.
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Purity
> 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LH CVGDTYPSND RCCHECRPGN GMVSRCSRSQ NTVCRPCGPG FYNDVVSSKP CKPCTWCNLR SGSERKQLCT ATQDTVCRCR AGTQPLDSYK PGVDCAPCPP GHFSPGDNQA CKPWTNCTLA GKHTLQPASN SSDAICEDRD PPATQPQETQ GPPARPITVQ PTEAWPRTSQ GPSTRPVEVP GGRAVAP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK -
Predicted molecular weight
47 kDa including tags -
Amino acids
29 to 216 -
Tags
Fc tag C-Terminus -
Additional sequence information
ab221340 has a Human IgG1 Fc tag at the C-terminus.
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Specifications
Our Abpromise guarantee covers the use of ab221340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
ELISA
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Form
Lyophilized -
Additional notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, 0.87% Sodium chloride, 0.44% L-Arginine
Lyophilized from 0.22 µm filtered solutionThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- ACT 35
- ACT35
- ACT35 antigen
see all -
Function
Receptor for TNFSF4/OX40L/GP34. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
Images
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Immobilized Recombinant human OX40L/TNFSF4 protein (His tag) (ab221412) at 5 μg/mL (100 μL/well) can bind ab221340 with a linear range of 2-25 ng/mL.
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FACS assay shows that recombinant Human OX40, Fc Tag can bind to 293T cell overexpressing human OX40L. The concentration of OX40 used is 0.03 µg/ml.
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SDS PAGE analysis of ab221340. The gel was stained overnight with Coomassie Blue. Due to glycosylation, the protein migrates at 65 kDa under reducing conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab221340 has been referenced in 1 publication.
- Zheng B et al. Sendai virus-based immunoadjuvant in hydrogel vaccine intensity-modulated dendritic cells activation for suppressing tumorigenesis. Bioact Mater 6:3879-3891 (2021). PubMed: 33937591