The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Measured by its ability to enhance LPS-stimulated IL8 secretion by THP1 human acute monocytic leukemia cells.
The ED 50 for this effect is typically 0.5-4 µg/mL.
< 1.000 Eu/µg
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.00 Constituents: 5% Trehalose, 0.75% Glycine, 0.6% Tris
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Monocyte differentiation antigen CD14
Monocyte differentiation antigen CD14 urinary form
Monocyte differentiation antigen CD14, membrane-bound form
Myeloid cell specific leucine rich glycoprotein
Myeloid cell-specific leucine-rich glycoprotein
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Contains 11 LRR (leucine-rich) repeats.
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.