Recombinant Human CD146 protein (ab114180)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human CD146 protein (ab114180)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: WB, SDS-PAGE, ELISA

    Description

    • Product name

      Recombinant Human CD146 protein
      See all CD146 proteins and peptides

    • Expression system

      Wheat germ

    • Accession

      P43121

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGLPRLVCAFLLAACCCCPRVAGVPGEAEQPAPELVEVEVGSTALLKCGL SQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGAT LALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPV NSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSG LYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTE KVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTN DNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP AAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKRE AGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNL SCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECT ASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTER KLPEPESRGVVIVAVIVCILVLAVLGAVLYFLYKKGKLPCRRSGKQEITL PPSRKSELVVEVKSDKLPEEMGLLQGSSGDKRAPGDQGEKYIDLRH

      • Predicted molecular weight

        97 kDa including tags

      • Amino acids

        1 to 646

    Specifications

    Our Abpromise guarantee covers the use of ab114180 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • A32 antigen
      • CD 146
      • CD146
      • CD146 antigen
      • Cell surface glycoprotein MUC18
      • Cell surface glycoprotein P1H12
      • Gicerin
      • Mcam
      • Melanoma adhesion molecule
      • Melanoma associated antigen A32
      • Melanoma associated antigen MUC18
      • Melanoma associated glycoprotein MUC18
      • Melanoma cell adhesion molecule
      • Melanoma-associated antigen A32
      • Melanoma-associated antigen MUC18
      • MelCAM
      • MUC 18
      • MUC18
      • MUC18_HUMAN
      • S endo 1
      • S endo 1 endothelial associated antigen
      • S-endo 1 endothelial-associated antigen
      see all

    • Function

      Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

    • Tissue specificity

      Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CD146 protein (ab114180)
      SDS-PAGE - Recombinant Human CD146 protein (ab114180)
      ab114180 on a 12.5% SDS-PAGE Stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab114180? Please let us know so that we can cite the reference in this datasheet.

    ab114180 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

    Would like to set up an ELISA to detect CD146 in human serum. CD146 might be extracellular cleaved fragment, however.

    Read More
    Answer

    Thank you for your call yesterday and for your patience while I have looked into your enquiry.

    I haven't found anything specific regarding the molecular weight of CD146 in serum, but I have found some literature regarding a soluble form of CD146 which is most likely the form found in serum. I've attached one article describing the soluble form running around 105 kDa in SDS-PAGE, while the membrane-bound form normally runs around 120-130 kDa. Please note that there are severalglycosylation sitesso the protein might have variable molecular weight depending on glycosylation state.

    I hope this information will be useful, but please let me know if you have any questions or if there is anything else that we can do for you and I'll be happy to help.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com