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Would like to set up an ELISA to detect CD146 in human serum. CD146 might be extracellular cleaved fragment, however.
Asked on Apr 13 2012
Thank you for your call yesterday and for your patience while I have looked into your enquiry.
I haven't found anything specific regarding the molecular weight of CD146 in serum, but I have found some literature regarding a soluble form of CD146 which is most likely the form found in serum. I've attached one article describing the soluble form running around 105 kDa in SDS-PAGE, while the membrane-bound form normally runs around 120-130 kDa. Please note that there are severalglycosylation sitesso the protein might have variable molecular weight depending on glycosylation state.
I hope this information will be useful, but please let me know if you have any questions or if there is anything else that we can do for you and I'll be happy to help.
Answered on Apr 13 2012