Recombinant Human CD16 protein (denatured) (ab130057)

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SDS-PAGE - Recombinant Human CD16 protein (denatured) (ab130057)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human CD16 protein (denatured)
      See all CD16 proteins and peptides

    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P08637

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRT EDLPKAVVFL EPQWYRVLEK DSVTLKCQGA YSPEDNSTQW FHNESLISSQ ASSYFIDAAT VDDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKGRKY FHHNSDFYIP KATLKDSGSY FCRGLFGSKN VSSETVNITI TQGLAVSTIS SFFPPGYQ

      • Predicted molecular weight

        26 kDa including tags

      • Amino acids

        18 to 208

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab130057 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 6% Urea, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • CD 16
      • CD 16a
      • CD16
      • CD16a
      • CD16a antigen
      • CD16B
      • CD16b antigen
      • Fc fragment of IgG
      • Fc fragment of IgG low affinity IIIa receptor
      • Fc fragment of IgG low affinity IIIa receptor (CD16)
      • Fc fragment of IgG receptor IIIa
      • Fc fragment of IgG, low affinity III, receptor (CD16)
      • Fc fragment of IgG, low affinity III, receptor for (CD16)
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16)
      • Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
      • Fc fragment of IgG, low affinity IIIa, receptor for
      • Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
      • Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
      • Fc gamma R3
      • Fc gamma receptor III 2 (CD 16)
      • Fc gamma receptor III A
      • Fc gamma receptor IIIA
      • Fc gamma receptor IIIb (CD 16)
      • Fc gamma RIII
      • Fc gamma RIII alpha
      • Fc gamma RIII beta
      • Fc gamma RIIIa
      • Fc gamma RIIIb
      • Fc of IgG
      • Fc-gamma receptor III2 (CD 16)
      • Fc-gamma receptor III2 (CD16)
      • Fc-gamma receptor IIIb (CD16)
      • Fc-gamma RIII
      • Fc-gamma RIII-alpha
      • Fc-gamma RIIIa
      • FCG 3
      • FCG3
      • FCG3A_HUMAN
      • FCgammaRIIIA
      • FCGR 3
      • FCGR 3A
      • FCGR3
      • FCGR3A
      • FCGR3A protein
      • FCGRIII
      • FCGRIII-2
      • FcR 10
      • FcR-10
      • FcR10
      • FcRIII
      • FcRIIIa
      • IGFR 3
      • IGFR3
      • IgG Fc receptor III 1
      • IgG Fc receptor III 2
      • IgG Fc receptor III-2
      • IMD20
      • immunoglobulin G Fc receptor III
      • immunoglobulin G Fc receptor III-2
      • Low affinity IIIa receptor
      • Low affinity immunoglobulin gamma Fc region receptor III A
      • Low affinity immunoglobulin gamma Fc region receptor III-A
      • Low affinity immunoglobulin gamma Fc region receptor IIIB
      • neutrophil-specific antigen NA
      see all

    • Function

      Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

    • Tissue specificity

      Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.

    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    • Post-translational
      modifications

      Glycosylated. Contains high mannose- and complex-type oligosaccharides.
      The soluble form is produced by a proteolytic cleavage.

    • Cellular localization

      Cell membrane. Secreted. Exists also as a soluble receptor.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CD16 protein (denatured) (ab130057)
      SDS-PAGE - Recombinant Human CD16 protein (denatured) (ab130057)
      15% SDS-PAGE showing ab130057 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab130057? Please let us know so that we can cite the reference in this datasheet.

    ab130057 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    wants to distinguish between CD16A and CD16B by ELISA
    ab89207: How was specificity for CD16B tested? Data?
    will try ab89207 in indirect ELISA - send testing discount code
    send ELISA protocol link
    ab130057: is protein CD16a or CD16b?

    Read More
    Answer

    ab130057is representing CD16a.

    I hope this is helpful. Please contact us again if you have any further questions.

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    Question

    wants to distinguish between CD16A and CD16B by ELISA
    ab89207: How was specificity for CD16B tested? Data?
    will try ab89207 in indirect ELISA - send testing discount code
    send ELISA protocol link
    ab130057: is protein CD16a or CD16b?

    Read More
    Answer

    Thank you for your phone call.

    I have contacted the lab for the information you had requested, and will let you know as soon asI hear back.
    I am sending you here the testing discount code for trying ab89207 in ELISA. Please see below.

    The protocol can be found here:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11389
    For your convenience I have it also attached to this email.


    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab89207 in ELISA. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ELISAand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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