Recombinant Human CD166 protein (Fc Chimera) (ab191959)
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Description
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Product name
Recombinant Human CD166 protein (Fc Chimera)
See all CD166 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKS VQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTI VKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVL HPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGP SGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPP PEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAI TVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSP SFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPS GLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISP EENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQA -
Predicted molecular weight
83 kDa including tags -
Amino acids
28 to 526 -
Additional sequence information
Fused with Fc fragment of Human IgG1 at the C-terminus (NP_001618).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab191959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilised -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.61% Tris, 5% Trehalose, 0.75% Glycine, L-Arginine, Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5% -
ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
General Info
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Alternative names
- Activated leukocyte cell adhesion molecule
- ALCAM
- ALCAM protein
see all -
Function
Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. -
Tissue specificity
Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Domain
The CD6 binding site is located in the N-terminal Ig-like domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human CD166 protein (Fc Chimera) (ab191959)SDS-PAGE analysis of reduced ab191959 stained overnight with Coomassie Blue. DTT-reduced protein migrates as 95-120 kDa due to glycosylation.
Datasheets and documents
References
ab191959 has not yet been referenced specifically in any publications.
