Recombinant Human CD166 protein (Fc Chimera) (ab191959)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human CD166 protein (Fc Chimera)
See all CD166 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKS VQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTI VKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVL HPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGP SGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPP PEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAI TVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSP SFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPS GLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISP EENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQA -
Predicted molecular weight
83 kDa including tags -
Amino acids
28 to 526 -
Additional sequence information
Fused with Fc fragment of Human IgG1 at the C-terminus (NP_001618).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab191959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 5% Trehalose, 0.75% Glycine, L-Arginine, Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5% -
ReconstitutionReconstitute with sterile deionized water to a concentration of 300 µg/ml.
General Info
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Alternative names
- Activated leukocyte cell adhesion molecule
- ALCAM
- ALCAM protein
see all -
Function
Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. -
Tissue specificity
Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Domain
The CD6 binding site is located in the N-terminal Ig-like domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human CD166 protein (Fc Chimera) (ab191959)
SDS-PAGE analysis of reduced ab191959 stained overnight with Coomassie Blue. DTT-reduced protein migrates as 95-120 kDa due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab191959 has not yet been referenced specifically in any publications.
