Overview

Description

  • Nature

    Recombinant
  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      PEEPLVVKVE EGDNAVLQCL KGTSDGPTQQ LTWSRESPLK PFLKLSLGLP GLGIHMRPLA IWLFIFNVSQ QMGGFYLCQP GPPSEKAWQP GWTVNVEGSG ELFRWNVSDL GGLGCGLKNR SSEGPSSPSG KLMSPKLYVW AKDRPEIWEG EPPCLPPRDS LNQSLSQDLT MAPGSTLWLS CGVPPDSVSR GPLSWTHVHP KGPKSLLSLE LKDDRPARDM WVMETGLLLP RATAQDAGKY YCHRGNLTMS FHLEITARPV LWHWLLRTGG WK
    • Molecular weight

      32 kDa including tags
    • Amino acids

      20 to 291
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Extracellular domain.

Specifications

Our Abpromise guarantee covers the use of ab234966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its binding ability in a functional ELISA.

    Immobilized Mouse anti-CD19 antibody (FMC63) at 2 μg/ml (100 μl/well) can bind ab234966 with a linear range of 10-78 ng/ml.

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level

    <=1.000 Eu/µg
  • Purity

    > 90 % SDS-PAGE.

  • Form

    Lyophilised
  • Additional notes

    Endotoxin Level determined by LAL method.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..

    pH: 7.40
    Constituents: 5% Trehalose, PBS

    Lyophilized from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.

General Info

  • Alternative names

    • Antibody deficiency due to defect in CD19
    • Antibody deficiency due to defect in CD19, included
    • AW495831
    • B lymphocyte antigen CD19
    • B lymphocyte surface antigen B4
    • B-lymphocyte antigen CD19
    • B-lymphocyte surface antigen B4
    • B4
    • CD19
    • CD19 antigen
    • CD19 molecule
    • Cd19 protein
    • CD19_HUMAN
    • CVID3
    • Differentiation antigen CD19
    • Leu 12
    • Leu-12
    • Leu12
    • MGC109570
    • MGC12802
    • T-cell surface antigen Leu-12
    see all
  • Function

    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in disease

    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Captured Human CD19 (20-291), His Tag on CM5 Chip via anti-His antibody, can bind FMC63 (mouse lgG2a) with an affinity constant of 2.01 nM as determined in SPR assay (Biacore T200 ).
  • Immobilized FMC63 MAb at 2 μg/mL (100 μL/well) binds ab234699.

    Linear range: 10-78 ng/mL (QC tested).

  • ab234966 at 2 μg/mL (100 μL/well) on Nickel Coated Plate, binds FMC63 (Mouse IgG2a).

    Linear range: 2-13 ng/mL (Routinely tested).

  • ab234966 at 0.1 μg/mL (100 μL/well) on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, binds FMC63 (Mouse IgG2a).

    Linear range: 0.6-10 ng/mL (Routinely tested).

  • FMC63 MAb (mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface, binds ab234966. 

    Affinity constant of 2.95 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Reduced ab234966 on SDS-PAGE, stained overnight with Coomassie Blue.

    Purity of protein >90%

    The protein migrates as 45-50 kDa under reducing conditions due to glycosylation.

  • 293 cells were transfected with FMC63-scFv and RFP tag.

    2x105 of the cells were first incubated with:

    A. His Tag-protein control.

    B. 10 ug/ml ab234966

    C. 10 ug/ml ab234966 and FMC63 (Mouse anti-CD19 antibody).

    The FITC Anti-6xHis tag antibody was used to analyse with FACS.

    RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity ofab234966.

References

ab234966 has not yet been referenced specifically in any publications.

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