Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human CD19 protein (Active)
See all CD19 proteins and peptides
Measured by its binding ability in a functional ELISA.
Immobilized Mouse anti-CD19 antibody (FMC63) at 2 μg/ml (100 μl/well) can bind ab234966 with a linear range of 10-78 ng/ml.
Purity> 90 % SDS-PAGE.
Endotoxin level<=1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequencePEEPLVVKVE EGDNAVLQCL KGTSDGPTQQ LTWSRESPLK PFLKLSLGLP GLGIHMRPLA IWLFIFNVSQ QMGGFYLCQP GPPSEKAWQP GWTVNVEGSG ELFRWNVSDL GGLGCGLKNR SSEGPSSPSG KLMSPKLYVW AKDRPEIWEG EPPCLPPRDS LNQSLSQDLT MAPGSTLWLS CGVPPDSVSR GPLSWTHVHP KGPKSLLSLE LKDDRPARDM WVMETGLLLP RATAQDAGKY YCHRGNLTMS FHLEITARPV LWHWLLRTGG WK
Predicted molecular weight32 kDa including tags
Amino acids20 to 291
TagsHis tag C-Terminus
Additional sequence informationExtracellular domain.
Our Abpromise guarantee covers the use of ab234966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin Level determined by LAL method.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
Constituents: 5% Trehalose, PBS
Lyophilized from 0.22 µm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
- Antibody deficiency due to defect in CD19
- Antibody deficiency due to defect in CD19, included
FunctionAssembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Involvement in diseaseDefects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
modificationsPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
- Information by UniProt
Immobilized FMC63 MAb at 2 μg/mL (100 μL/well) binds ab234699.
Linear range: 10-78 ng/mL (QC tested).
ab234966 at 2 μg/mL (100 μL/well) on Nickel Coated Plate, binds FMC63 (Mouse IgG2a).
Linear range: 2-13 ng/mL (Routinely tested).
ab234966 at 0.1 μg/mL (100 μL/well) on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, binds FMC63 (Mouse IgG2a).
Linear range: 0.6-10 ng/mL (Routinely tested).
Captured Human CD19 (20-291), His Tag on CM5 Chip via anti-His antibody, can bind FMC63 (mouse lgG2a) with an affinity constant of 2.01 nM as determined in SPR assay (Biacore T200 ).
FMC63 MAb (mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface, binds ab234966.
Affinity constant of 2.95 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Reduced ab234966 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >90%
The protein migrates as 45-50 kDa under reducing conditions due to glycosylation.
293 cells were transfected with FMC63-scFv and RFP tag.
2x105 of the cells were first incubated with:
A. His Tag-protein control.
B. 10 ug/ml ab234966
C. 10 ug/ml ab234966 and FMC63 (Mouse anti-CD19 antibody).
The FITC Anti-6xHis tag antibody was used to analyse with FACS.
RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity ofab234966.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234966 has not yet been referenced specifically in any publications.