Bioactive grade

Recombinant human CD19 protein (Active) (Biotin) (ab167717)

Description

  • Product name

    Recombinant human CD19 protein (Active) (Biotin)
    See all CD19 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA.


    Immobilized FMC63 mAb at 2 μg/mL (100 μL/well) binds Biotinylated Human CD19, Fc tag, with a linear range of 0.15-2.5 μg/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      LFLTPMEVRP EEPLVVKVEE GDNAVLQCLK GTSDGPTQQL TWSRESPLKP FLKLSLGLPG LGIHMRPLAI WLFIFNVSQQ MGGFYLCQPG PPSEKAWQPG WTVNVEGSGE LFRWNVSDLG GLGCGLKNRS SEGPSSPSGK LMSPKLYVWA KDRPEIWEGE PPCLPPRDSL NQSLSQDLTM APGSTLWLSC GVPPDSVSRG PLSWTHVHPK GPKSLLSLEL KDDRPARDMW VMETGLLLPR ATAQDAGKYY CHRGNLTMSF HLEITARPVL WHWLLRTGGW K
    • Predicted molecular weight

      56 kDa including tags
    • Amino acids

      20 to 291
    • Tags

      Fc tag C-Terminus
    • Additional sequence information

      A Chimeric Protein: Biotinylated CD19-Fc is fused with Fc fragment of human IgG1 at the C-terminus.
  • Conjugation

    Biotin

Specifications

Our Abpromise guarantee covers the use of ab167717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    The biotin to protein ratio is 2.5-3.5 as determined by the HABA assay.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in 50µl sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.

General Info

  • Alternative names

    • Antibody deficiency due to defect in CD19
    • Antibody deficiency due to defect in CD19, included
    • AW495831
    • B lymphocyte antigen CD19
    • B lymphocyte surface antigen B4
    • B-lymphocyte antigen CD19
    • B-lymphocyte surface antigen B4
    • B4
    • CD19
    • CD19 antigen
    • CD19 molecule
    • Cd19 protein
    • CD19_HUMAN
    • CVID3
    • Differentiation antigen CD19
    • Leu 12
    • Leu-12
    • Leu12
    • MGC109570
    • MGC12802
    • T-cell surface antigen Leu-12
    see all
  • Function

    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in disease

    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Immobilized Anti-Mouse FMC63 Mab at 2 μg/mL (100 μL/well) binds ab167717.

    Linear range: 0.039-0.625 μg/mL (QC tested).

  • Immobilized Anti-Mouse FMC63 Mab at 2 μg/mL (100 μL/well) binds ab 167717.

    Linear range: 0.039-0.625 μg/mL (QC tested).

  • Reduced ab167717 on SDS-PAGE, stained overnight with Coomassie Blue.

    Purity of protein >95%.

    The protein migrates as 56-66 kDa under reducing conditions, due to glycosylation.

  • HEK293 cells were transfected with FCM63-scFv and RFP tag . 2x105 of the cells were first incubated with:

    A. Biotinylated protein control.

    B. 10 μg/ml of ab167717.

    C. 10 μg/ml of ab167717 and FMC63(Mouse anti-CD19 antibody). FITC Streptavidin was used to analyse with FACS. RFP was used to evaluate CAR(FMC63-scFv) expression and FITC was used to evaluate the binding activity of ab167717.

  • Human T cells were lentivirally transduced with anti-CD19 CAR and cultured for 11 days.

    Eleven days post-transduction, 1e6 cells were stained for the expression of CD3 and anti-CD19 CAR with FITC anti-human CD3 antibody and ab167717, followed by PE-conjugated streptavidin, respectively.

    A. Non-transduced T cells were used as a control for gating of CAR expression. (Data are kindly provided by Beijing Bowei Huaen Medical Technology Co. Ltd.)

References

ab167717 has not yet been referenced specifically in any publications.

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