Recombinant Human CD22 protein (Tagged) (Biotin) (ab271414)

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SDS-PAGE - Recombinant Human CD22 protein (Tagged) (Biotin) (ab271414)
  • Size Exclusion Chromatography - Recombinant Human CD22 protein (Tagged) (Biotin) (ab271414)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: >= 90% SDS-PAGE
  • Tags: Avi tag C-Terminus, Fc tag C-Terminus
  • Suitable for: SDS-PAGE, Size Exclusion Chromatography

Description

  • Product name

    Recombinant Human CD22 protein (Tagged) (Biotin)
    See all CD22 proteins and peptides

  • Purity

    >= 90 % SDS-PAGE.

  • Expression system

    HEK 293 cells

  • Accession

    CD22

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      KNCTLSIHPV HLNDSGQLGL RMESKTEKWM ERIHLNVSER PFPPHIQLPP EIQESQEVTL TCLLNFSCYG YPIQLQWLLE GVPMRQAAVT STSLTIKSVF TRSELKFSPQ WSHHGKIVTC QLQDADGKFL SNDTVQLNVK HTPKLEIKVT PSDAIVREGD SVTMTCEVSS SNPEYTTVSW LKDGTSLKKQ NTFTLNLREV TKDQSGKYCC QVSNDVGPGR SEEVFLQVQY A

    • Predicted molecular weight

      104 kDa including tags

    • Molecular weight information

      This protein runs at a higher MW by SDS-PAGE due to glycosylation.

    • Amino acids

      100 to 330

    • Modifications

      biotinylated

    • Tags

      Avi tag C-Terminus , Fc tag C-Terminus

    • Additional sequence information

      AviTag™ fused to Fc region of Human IgG1. Genbank: NM_001771

  • Conjugation

    Biotin

Specifications

Our Abpromise guarantee covers the use of ab271414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Size Exclusion Chromatography

  • Form

    Liquid

  • Additional notes

    Enzymatically biotin-labeled using Avi-tag™ technology

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.

    pH: 7.40
    Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)

General Info

  • Alternative names

    • B cell receptor CD22 precursor
    • B lymphocyte cell adhesion molecule
    • B-cell receptor CD22
    • B-lymphocyte cell adhesion molecule
    • BL CAM
    • BL-CAM
    • BLCAM
    • CD 22
    • CD22
    • CD22 antigen
    • CD22 molecule
    • CD22 protein
    • CD22_HUMAN
    • Lectin 2
    • Leu14
    • Lyb8
    • MGC130020
    • sialic acid binding Ig like lectin 2
    • Sialic acid binding immunoglobulin like lectin 2
    • Sialic acid-binding Ig-like lectin 2
    • SIGLEC 2
    • Siglec-2
    • SIGLEC2
    • T cell surface antigen Leu 14
    • T-cell surface antigen Leu-14
    see all

  • Function

    Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

  • Tissue specificity

    B-lymphocytes.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Domain

    Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

  • Post-translational
    modifications

    Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
    Phosphorylated on tyrosine residues by LYN.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human CD22 protein (Tagged) (Biotin) (ab271414)
    SDS-PAGE - Recombinant Human CD22 protein (Tagged) (Biotin) (ab271414)

    SDS-PAGE analysis of ab271414. This protein runs at a higher molecular weight due to glycosylation.

  • Size Exclusion Chromatography - Recombinant Human CD22 protein (Tagged) (Biotin) (ab271414)
    Size Exclusion Chromatography - Recombinant Human CD22 protein (Tagged) (Biotin) (ab271414)

    SEC analysis of ab271414.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab271414? Please let us know so that we can cite the reference in this datasheet.

ab271414 has not yet been referenced specifically in any publications.

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