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Bioactive grade

Recombinant human CD226 protein (Active) (ab219701)

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SDS-PAGE - Recombinant human CD226 protein (Active) (ab219701)
  • Functional Studies - Recombinant human CD226 protein (Active) (ab219701)
  • Functional Studies - Recombinant human CD226 protein (Active) (ab219701)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human CD226 protein (Active)
    See all CD226 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA.

    Immobilized ab219701 at 2 μg/mL (100 μL/well) can bind Human CD155, Fc tag with a linear range of 3.3-51.2 ng/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

    Q15762
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      EEVLWHTSVPFAENMSLECVYPSMGILTQVEWFKIGTQQDSIAIFSPTHG MVIRKPYAERVYFLNSTMASNNMTLFFRNASEDDVGYYSCSLYTYPQGTW QKVIQVVQSDSFEAAVPSNSHIVSEPGKNVTLTCQPQMTWPVQAVRWEKI QPRQIDLLTYCNLVHGRNFTSKFPRQIVSNCSHGRWSVIVIPDVTVSDSG LYRCYLQASAGENETFVMRLTVAEGKTDN
    • Predicted molecular weight

      28 kDa including tags
    • Amino acids

      19 to 247
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (NP_006557).

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab219701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

    Lyophilized from 0.22 µm filtered solution.
    Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

General Info

  • Alternative names

    • adhesion glycoprotein
    • CD226
    • CD226 antigen
    • CD226 molecule
    • CD226_HUMAN
    • DNAM 1
    • DNAM-1
    • DNAM1
    • DNAX accessory molecule 1
    • platelet and T cell activation antigen 1
    • PTA1
    • T lineage specific activation antigen 1 antigen
    • TLiSA1
    see all
  • Function

    Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
  • Tissue specificity

    Expressed by peripheral blood T-lymphocytes.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q15762 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human CD226 protein (Active) (ab219701)
    SDS-PAGE - Recombinant human CD226 protein (Active) (ab219701)

    SDS-PAGE analysis of reduced ab219701 stained overnight with Coomassie Blue.

  • Functional Studies - Recombinant human CD226 protein (Active) (ab219701)
    Functional Studies - Recombinant human CD226 protein (Active) (ab219701)

    Ab219701 at 3µg/ml can bind to 293T cell overexpressing human CD155 as dtermined by FACS assay.

  • Functional Studies - Recombinant human CD226 protein (Active) (ab219701)
    Functional Studies - Recombinant human CD226 protein (Active) (ab219701)

    Immobilized ab219701 at 2 μg/mL (100 μL/well) can bind Human CD155, Fc tag with a linear range of 3.3-51.2 ng/mL.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab219701? Please let us know so that we can cite the reference in this datasheet.

    ab219701 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab219701.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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