Bioactive grade

Recombinant human CD252 protein (His tag) (ab221412)

Description

  • Product name

    Recombinant human CD252 protein (His tag)
    See all CD252 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized ab221412 at 5 μg/ml (100 μl/well) can bind Human OX40, Fc Tag with a linear range of 0.2-5 ng/ml.

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      QVSHRYPRIQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGF YLISLKGYFSQEVNISLHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVY LNVTTDNTSLDDFHVNGGELILIHQNPGEFCVL
    • Predicted molecular weight

      17 kDa including tags
    • Amino acids

      51 to 183
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Extracellular domain (NP_003317).

Specifications

Our Abpromise guarantee covers the use of ab221412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD 134L
    • CD 252
    • CD134
    • CD134 ligand
    • CD134L
    • CD252
    • CD252 antigen
    • glycoprotein 34 kd
    • Glycoprotein Gp34
    • GP34
    • OX-40L
    • OX40 antigen ligand
    • OX40 ligand
    • OX40L
    • TAX transcriptionally-activated glycoprotein 1
    • TNFL4_HUMAN
    • Tnfsf4
    • Tumor necrosis factor (ligand) superfamily member 4
    • Tumor necrosis factor ligand superfamily member 4
    • TXGP1
    see all
  • Function

    Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
  • Involvement in disease

    Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
  •  A SEC-HPLC analysis showing over 90% of Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) present as active trimers with a molecular mass around 90 kDa.

  • FACS assay shows that Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) can bind to 293T cell overexpressing human OX40. The concentration of OX40 Ligand used is 0.1 µg/mL.
  • Immobilized Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) at 2 µg/mL (100 µL/well) can bind Biotinylated Human OX40, Fc,Avitag with a linear range of 10-78 ng/mL.
  • Immobilized Biotinylated Human OX40, Fc,Avitag at 2 µg/mL (100 µL/well) on streptavidin precoated (0.5 µg/well) plate, can bind Human OX40 Ligand, His Tag (active trimer) (HPLC-verified) with a linear range of 5-39 ng/mL.
  • SDS-PAGE analysis of reduced ab221412 stained overnight with Coomassie Blue.

    The protein migrates as 25-30 kDa under reducing conditions (SDS-PAGE) due to glycosylation.

References

ab221412 has not yet been referenced specifically in any publications.

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