Recombinant human CD27 protein (His tag) (ab219420)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 85% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant human CD27 protein (His tag)
See all CD27 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7. The ED50 range ≤ 0.5 µg/ml.
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Purity
> 85 % SDS-PAGE.
Affinity purified -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ADPTPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIP GVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKEC TECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFR QLPARTLSTHWPPQRSLCSSDFIRLEPKSCDKTHTCPPCPAPELLGGPSV FLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK PREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK GQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENN YKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGKHHHHHH -
Predicted molecular weight
46 kDa including tags -
Amino acids
21 to 191 -
Tags
His tag C-Terminus -
Additional sequence information
Fused to hIgG-His-tag at C-terminus (NP_001233).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab219420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CD 27
- CD27
- CD27 antigen
see all -
Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
Tissue specificity
Found in most T-lymphocytes. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219420 has not yet been referenced specifically in any publications.