Key features and details
- Expression system: HEK 293 cells
- Purity: > 92% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human CD272/BTLA protein (Fc Chimera Active)
See all CD272/BTLA proteins and peptides
Measured by its binding ability in a functional ELISA. Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind ab219719 with a linear range of 0.02-1.25 μg/mL.
Purity> 92 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceKESCDVQLYI KRQSEHSILA GDPFELECPV KYCANRPHVT WCKLNGTTCV KLEDRQTSWK EEKNISFFIL HFEPVLPNDN GSYRCSANFQ SNLIESHSTT LYVT
Predicted molecular weight39 kDa including tags
Amino acids31 to 134
TagsFc tag C-Terminus
Additional sequence informationFused with a human IgG1 Fc tag at the C terminus (Accession # NP_001078826.1)
Our Abpromise guarantee covers the use of ab219719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as CD272
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.61% Tris buffer, 0.75% Glycine, L-Arginine, Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
- B and T lymphocyte associated protein
- B and T lymphocyte attenuator
- B and T lymphocyte associated
FunctionLymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPhosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219719 has not yet been referenced specifically in any publications.