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    recombinant-human-cd28-protein-active-ab286013.pdf

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Immunology Adaptive Immunity B Cells CD
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Recombinant human CD28 protein (Active) (ab286013)

  • Datasheet
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ELISA - Recombinant human CD28 protein (Active) (ab286013)
  • SDS-PAGE - Recombinant human CD28 protein (Active) (ab286013)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: >= 95% SDS-PAGE
  • Endotoxin level: < 0.200 Eu/µg
  • Active: Yes
  • Tags: Fc tag C-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human CD28 protein (Active)
    See all CD28 proteins and peptides
  • Biological activity

    Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 µg/mL, 100 µL/well with a linear range of 0.03 – 0.81 µg/mL.

  • Purity

    >= 95 % SDS-PAGE.

  • Endotoxin level

    < 0.200 Eu/µg
  • Expression system

    HEK 293 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      25 kDa
    • Molecular weight information

      66-70 kDa
    • Tags

      Fc tag C-Terminus
    • Additional sequence information

      Source HEK 293 cells

Specifications

Our Abpromise guarantee covers the use of ab286013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilized
  • Additional notes

    This product is manufactured by BioVision, an Abcam company and was previously called 9237 Human CellExp™ CD28, Human Recombinant. 9237-100 is the same size as the 100 µg size of ab286013.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile PBS, pH 7.4 to a concentration of 10 µg/ml

General Info

  • Alternative names

    • CD 28
    • CD28
    • CD28 antigen
    • CD28 molecule
    • CD28_HUMAN
    • MGC138290
    • T cell antigen CD28
    • T cell specific surface glycoprotein
    • T cell specific surface glycoprotein CD28
    • T-cell-specific surface glycoprotein CD28
    • TP44
    see all
  • Function

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificity

    Expressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P10747 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • ELISA - Recombinant human CD28 protein (Active) (ab286013)
    ELISA - Recombinant human CD28 protein (Active) (ab286013)

    CD28 and CD80-Biotin ELISA binding activity:

    Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 µg/mL, 100 ng/well with a linear range of 0.03 – 0.81 µg/mL.

  • SDS-PAGE - Recombinant human CD28 protein (Active) (ab286013)
    SDS-PAGE - Recombinant human CD28 protein (Active) (ab286013)

    SDS-PAGE (4-20%): Human CD28, Fc-Tag protein was run on SDS PAGE gel under reducing (R) conditions and stained with Coomassie Blue.

    Lane M: Protein Marker

    Lane 1: Biotinylated Human CD28 Fc Tag (2 µg)

    Lane 2: Human CD28 Fc Tag (2 µg).

    The proteins migrate to around 55 kDa due to glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab286013? Please let us know so that we can cite the reference in this datasheet.

ab286013 has not yet been referenced specifically in any publications.

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