Recombinant human CD28 protein (Active) (ab286013)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.200 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human CD28 protein (Active)
See all CD28 proteins and peptides -
Biological activity
Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 µg/mL, 100 µL/well with a linear range of 0.03 – 0.81 µg/mL.
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Purity
>= 95 % SDS-PAGE. -
Endotoxin level
< 0.200 Eu/µg -
Expression system
HEK 293 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
25 kDa -
Molecular weight information
66-70 kDa -
Tags
Fc tag C-Terminus -
Additional sequence information
Source HEK 293 cells
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Specifications
Our Abpromise guarantee covers the use of ab286013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 9237 Human CellExp™ CD28, Human Recombinant. 9237-100 is the same size as the 100 µg size of ab286013.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in sterile PBS, pH 7.4 to a concentration of 10 µg/ml
General Info
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Alternative names
- CD 28
- CD28
- CD28 antigen
see all -
Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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ELISA - Recombinant human CD28 protein (Active) (ab286013)
CD28 and CD80-Biotin ELISA binding activity:
Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 µg/mL, 100 ng/well with a linear range of 0.03 – 0.81 µg/mL.
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SDS-PAGE - Recombinant human CD28 protein (Active) (ab286013)SDS-PAGE (4-20%): Human CD28, Fc-Tag protein was run on SDS PAGE gel under reducing (R) conditions and stained with Coomassie Blue.
Lane M: Protein Marker
Lane 1: Biotinylated Human CD28 Fc Tag (2 µg)
Lane 2: Human CD28 Fc Tag (2 µg).
The proteins migrate to around 55 kDa due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab286013 has not yet been referenced specifically in any publications.
