Bioactive grade

Recombinant human CD3 delta + CD3 epsilon (biotinylated ) protein (Active) (Biotin) (ab205994)

Description

  • Product name

    Recombinant human CD3 delta + CD3 epsilon (biotinylated ) protein (Active) (Biotin)
    See all CD3 delta + CD3 epsilon proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA.

    Biotinylated Human CD3 delta and CD3 epsilon protein was chemically biotin labeled to primary amines without decreasing its bioactivity.

     

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Amino Acid Sequence 1

    • Species

      Human
    • Sequence

      DGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMD
    • Predicted molecular weight

      39 kDa including tags
    • Amino acids

      23 to 126
    • Modifications

      biotinylated
    • Tags

      His tag C-Terminus , Fc tag C-Terminus
    • Additional sequence information

      (NP_000724.1).
    • Amino Acid Sequence 2

    • Species

      Human
    • Sequence

      FKIPIEELED RVFVNCNTSI TWVEGTVGTL LSDITRLDLG KRILDPRGIY RCNGTDIYKD KESTVQVHYR MCQSCVELDP ATVA
    • Predicted molecular weight

      38 kDa including tags
    • Amino acids

      22 to 105
    • Modifications

      biotinylated
    • Tags

      DDDDK tag C-Terminus , Fc tag C-Terminus
    • Additional sequence information

      (AAH39035).
  • Conjugation

    Biotin

Specifications

Our Abpromise guarantee covers the use of ab205994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Additional notes

    The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    Lyophilised from a 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilised protein in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.

General Info

  • Alternative names

    • CD3 antigen delta subunit
    • CD3 delta
    • CD3d
    • CD3d antigen delta polypeptide (TiT3 complex)
    • CD3d molecule delta (CD3 TCR complex)
    • CD3e
    • CD3e antigen epsilon polypeptide (TiT3 complex)
    • CD3e molecule epsilon (CD3 TCR complex)
    • CD3E_HUMAN
    • IMD18
    • IMD19
    • OKT3 delta chain
    • T cell antigen receptor complex epsilon subunit of T3
    • T cell receptor T3 delta chain
    • T cell surface glycoprotein CD3 delta chain
    • T-cell surface antigen T3/Leu-4 epsilon chain
    • T-cell surface glycoprotein CD3 epsilon chain
    • T3D
    • T3E
    • TCRE
    see all
  • Function

    The CD3 complex mediates signal transduction.
  • Involvement in disease

    Immunodeficiency 18
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • ab205994 binds OKT3.

    Linear range: 2-39 ng/mL.

    ab205994 is coated at 1 ug/mL (100 μL/well), on a streptavidin coated plates (QC tested).

  • Immobilized SP34-2 at 5 μg/mL (100 μL/well) binds ab205994. 

    Linear range: 1-20 ng/mL (Routinely tested).

  • Reduced ab205994 on SDS-PAGE, stained overnight with Coomassie Blue.

    Purity of protein >95%.

    The protein migrates as 45-55 kDa under reducing condition due to glycosylation.

References

ab205994 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205994.
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