Recombinant human CD3 epsilon + CD3D protein (Active) (ab286041)
Key features and details
- Expression system: Expi293 F cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, ELISA, Functional Studies
Description
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Product name
Recombinant human CD3 epsilon + CD3D protein (Active) -
Biological activity
Human CD3 epsilon and CD3 delta Heterodimer Protein was immobilized at 0.5 µg/ml (100 µl/well). Dose response curve using Anti-hCD3E/CD3D Ab foralumab gave an EC50 of 21.69 ng/ml by ELISA. Human CD3 epsilon and CD3 delta Heterodimer Protein was Immobilized at 0.1 µg/ml (100 µl/Well). Dose response curve using Anti-hCD3E/CD3D Ab OKT3 gave an EC50 of 21 ng/ml by ELISA.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Expi293 F cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Predicted molecular weight
38 kDa -
Amino acids
23 to 126 -
Tags
Fc tag C-Terminus
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Amino Acid Sequence 2
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Species
Human -
Predicted molecular weight
35 kDa -
Amino acids
22 to 105 -
Tags
Fc tag C-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab286041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
ELISA
Functional Studies
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Form
Lyophilized -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called P1183 Human CellExp™ CD3 epsilon & CD3 delta Heterodimer. P1183-10 is the same size as the 10 µg size of ab286041.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 7.40
Constituent: 99% PBS
Lyophilized from 0.22 µm filtered solution in PBS pH 7.4.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in sterile deionized water to the desired protein concentration.
General Info
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Cellular localization
CD3 epsilon: Membrane. CD3D: Membrane.
Images
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ab286041 was Immobilized at 0.1 µg/ml (100 µl/Well). Dose response curve using Anti-hCD3E/CD3D Ab2 OKT3 gave an EC50 of 21 ng/ml by ELISA.
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ab286041 was immobilized at 0.5 µg/ml (100 µl/well). Dose response curve using Anti-hCD3E/CD3D Ab1 foralumab gave an EC50 of 21.69 ng/ml by ELISA
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ab286041 run on Tris-Bis PAGE gel under reducing conditions. The protein migrates as 40-50 kDa due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab286041 has not yet been referenced specifically in any publications.