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  1. Link

    recombinant-human-cd3-epsilon-protein-active-ab167755.pdf

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Immunology Adaptive Immunity T Cells CD
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Bioactive grade

Recombinant human CD3 epsilon protein (Active) (ab167755)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant human CD3 epsilon protein (Active) (ab167755)
  • Size Exclusion Chromatography - Recombinant human CD3 epsilon protein (Active) (ab167755)
  • Functional Studies - Recombinant human CD3 epsilon protein (Active) (ab167755)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human CD3 epsilon protein (Active)
    See all CD3 epsilon proteins and peptides
  • Biological activity

    Immobilized Mouse Anti-Human CD3 at 2 μg/mL (100 μL/well) binds ab167755.

    Linear range: 0.2-2 ng/mL (QC tested).

     

  • Purity

    > 90 % SDS-PAGE.
    >90% as determined by SEC-MALS.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

    P07766-1
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      DGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMD
    • Predicted molecular weight

      17 kDa including tags
    • Molecular weight information

      As a result of glycosylation, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
    • Amino acids

      23 to 126
    • Tags

      His tag C-Terminus
    • Additional sequence information

      As a result of glycosylation, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Associated products

  • Related Products

    • Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)
    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab167755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..

    pH: 7.54
    Constituent: 100% PBS

    Lyophilized from 0.22 µm filtered solution.
    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 600 µg/ml.

General Info

  • Alternative names

    • CD3 epsilon
    • CD3e
    • CD3e antigen
    • CD3E antigen epsilon polypeptide
    • CD3e antigen epsilon polypeptide (TiT3 complex)
    • CD3E antigen, epsilon subunit
    • CD3e molecule epsilon
    • CD3e molecule, epsilon (CD3 TCR complex)
    • CD3e molecule, epsilon (CD3-TCR complex)
    • CD3E_HUMAN
    • IMD18
    • T cell antigen receptor complex epsilon subunit of T3
    • T cell surface antigen T3/Leu 4 epsilon chain
    • T cell surface glycoprotein CD3 epsilon chain
    • T-cell surface antigen T3/Leu-4 epsilon chain
    • T-cell surface glycoprotein CD3 epsilon chain
    • T3E
    • TCRE
    see all
  • Function

    The CD3 complex mediates signal transduction.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P07766 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant human CD3 epsilon protein (Active) (ab167755)
    SDS-PAGE - Recombinant human CD3 epsilon protein (Active) (ab167755)

    ab167755 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Size Exclusion Chromatography - Recombinant human CD3 epsilon protein (Active) (ab167755)
    Size Exclusion Chromatography - Recombinant human CD3 epsilon protein (Active) (ab167755)

    The purity of Human CD3 epsilon, His Tag (ab167755) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

  • Functional Studies - Recombinant human CD3 epsilon protein (Active) (ab167755)
    Functional Studies - Recombinant human CD3 epsilon protein (Active) (ab167755)

    Immobilized Mouse Anti Human CD3 at 2 μg/mL (100 μL/well) can bind ab167755 with a linear range of 0.2-2 ng/mL (QC tested).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab167755? Please let us know so that we can cite the reference in this datasheet.

ab167755 has not yet been referenced specifically in any publications.

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