Recombinant human CD3 epsilon protein (Active) (ab167755)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CD3 epsilon protein (Active)
See all CD3 epsilon proteins and peptides -
Biological activity
Immobilized Mouse Anti-Human CD3 at 2 μg/mL (100 μL/well) binds ab167755.
Linear range: 0.4-6.25 ng/mL (QC tested).
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Purity
> 90 % SDS-PAGE.
Purified by Immobilized metal affinity chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMD -
Predicted molecular weight
13 kDa including tags -
Amino acids
23 to 126 -
Tags
His tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab167755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 600 µg/ml.
General Info
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Alternative names
- CD3 epsilon
- CD3e
- CD3e antigen
see all -
Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human CD3 epsilon protein (Active) (ab167755)
Immobilized Mouse Anti-Human CD3 at 2 μg/mL (100 μL/well) binds ab167755.
Linear range: 0.4-6.25 ng/mL (QC tested).
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Functional Studies - Recombinant human CD3 epsilon protein (ab167755)SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, binds ab167755 with an affinity constant of 0.97 nM , as determined in a SPR assay (Biacore T200) (Routinely tested).
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SDS-PAGE - Recombinant human CD3 epsilon protein (ab167755)Reduced ab167755 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >90%
The protein migrates as 15-16 kDa under reducing conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab167755 has not yet been referenced specifically in any publications.