Bioactive grade

Recombinant human CD3 epsilon protein (Active) (ab167755)

Description

  • Product name

    Recombinant human CD3 epsilon protein (Active)
    See all CD3 epsilon proteins and peptides
  • Biological activity

    Immobilized Mouse Anti-Human CD3 at 2 μg/mL (100 μL/well) binds ab167755.

    Linear range: 0.4-6.25 ng/mL (QC tested).

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      DGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMD
    • Predicted molecular weight

      13 kDa including tags
    • Amino acids

      23 to 126
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.
    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.

General Info

  • Alternative names

    • CD3 epsilon
    • CD3e
    • CD3e antigen
    • CD3E antigen epsilon polypeptide
    • CD3e antigen epsilon polypeptide (TiT3 complex)
    • CD3E antigen, epsilon subunit
    • CD3e molecule epsilon
    • CD3e molecule, epsilon (CD3 TCR complex)
    • CD3e molecule, epsilon (CD3-TCR complex)
    • CD3E_HUMAN
    • IMD18
    • T cell antigen receptor complex epsilon subunit of T3
    • T cell surface antigen T3/Leu 4 epsilon chain
    • T cell surface glycoprotein CD3 epsilon chain
    • T-cell surface antigen T3/Leu-4 epsilon chain
    • T-cell surface glycoprotein CD3 epsilon chain
    • T3E
    • TCRE
    see all
  • Function

    The CD3 complex mediates signal transduction.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Immobilized Mouse Anti-Human CD3 at 2 μg/mL (100 μL/well) binds ab167755.

    Linear range: 0.4-6.25 ng/mL (QC tested).

  • SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, binds ab167755 with an affinity constant of 0.97 nM , as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Reduced ab167755 on SDS-PAGE, stained overnight with Coomassie Blue.

    Purity of protein >90%

    The protein migrates as 15-16 kDa under reducing conditions.

References

ab167755 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab167755.
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