Recombinant human CD3 epsilon protein (Active) (ab167755)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CD3 epsilon protein (Active)
See all CD3 epsilon proteins and peptides -
Biological activity
Immobilized Mouse Anti-Human CD3 at 2 μg/mL (100 μL/well) binds ab167755.
Linear range: 0.2-2 ng/mL (QC tested).
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Purity
> 90 % SDS-PAGE.
>90% as determined by SEC-MALS. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DGNEEMGGIT QTPYKVSISG TTVILTCPQY PGSEILWQHN DKNIGGDEDD KNIGSDEDHL SLKEFSELEQ SGYYVCYPRG SKPEDANFYL YLRARVCENC MEMD -
Predicted molecular weight
17 kDa including tags -
Molecular weight information
As a result of glycosylation, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE). -
Amino acids
23 to 126 -
Tags
His tag C-Terminus -
Additional sequence information
As a result of glycosylation, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab167755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
pH: 7.54
Constituent: 100% PBS
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 600 µg/ml.
General Info
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Alternative names
- CD3 epsilon
- CD3e
- CD3e antigen
see all -
Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant human CD3 epsilon protein (Active) (ab167755)
ab167755 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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Size Exclusion Chromatography - Recombinant human CD3 epsilon protein (Active) (ab167755)The purity of Human CD3 epsilon, His Tag (ab167755) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
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Functional Studies - Recombinant human CD3 epsilon protein (Active) (ab167755)Immobilized Mouse Anti Human CD3 at 2 μg/mL (100 μL/well) can bind ab167755 with a linear range of 0.2-2 ng/mL (QC tested).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab167755 has not yet been referenced specifically in any publications.