Recombinant Human CD3 epsilon protein (Fc Chimera His Tag) (ab220590)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human CD3 epsilon protein (Fc Chimera His Tag)
See all CD3 epsilon proteins and peptides -
Biological activity
Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 μg/mL (100 μL/well) can bind ab220590 with a linear range of 0.1-3 ng/mL (Routinely tested).
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DGNEEMGG ITQTPYKVSI SGTTVILTCP QYPGSEILWQ HNDKNIGGDE DDKNIGSDED HLSLKEFSEL EQSGYYVCYP RGSKPEDANF YLYLRARVCE NCMEMD -
Predicted molecular weight
39 kDa including tags -
Amino acids
23 to 126 -
Tags
His tag C-Terminus , Fc tag C-Terminus -
Additional sequence information
Protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.(NP_000724.1). The protein migrates as 5415-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Description
Recombinant human CD3 epsilon protein (Fc Chimera His Tag)
Associated products
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Related Buffer
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab220590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.54
Constituent: 100% PBS
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- CD3 epsilon
- CD3e
- CD3e antigen
see all -
Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE analysis of reduced ab220590 stained overnight with Coomassie Blue.
The protein migrates as 44-54 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
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Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 μg/mL (100 μL/well) can bind ab220590 with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab220590 has not yet been referenced specifically in any publications.