Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
Product nameRecombinant human CD40 protein (Fc Chimera Active)
See all CD40 proteins and peptides
Activity: The ED50 of ab83917 is typically 1.0-1.5 ug/ml as measured by its ability to neutralize CD40 ligand mediated proliferation of T47-D cells.
Purity> 95 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceTheoretical Sequence EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDT WNR ETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVL HRSC SPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLV VQQAG TNKTDVVCGPQDRLRRSSNTKVDKKVEPKSCDKTHTCPPCPAPELLGG PSVFL FPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK PREEQ YNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP REPQVY TLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPV LDSDG SFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Amino acids21 to 193
Additional sequence informationFused with the Fc region of Human IgG1 at the C-terminus.
Our Abpromise guarantee covers the use of ab83917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: PBS, 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
- B cell associated molecule CD40
- B cell surface antigen CD40
FunctionReceptor for TNFSF5/CD40LG.
Tissue specificityB-cells and in primary carcinomas.
Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
Sequence similaritiesContains 4 TNFR-Cys repeats.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83917 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83917
Lane 2: ab83917 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83917 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
Anti-CD40 antibody (ab13545) at 1/1000 dilution +
Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab83917 has not yet been referenced specifically in any publications.