Recombinant Human CD43 protein (ab171717)

Submit a review Submit a question
SDS-PAGE - Recombinant Human CD43 protein (ab171717)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    Description

    • Product name

      Recombinant Human CD43 protein

    • Purity

      > 85 % SDS-PAGE.
      ab171717 was purified by anion-exchange and gel-filtration chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P16150

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSSTTAVQT PTSGEPLVST SEPLSSKMYT TSITSDPKAD STGDQTSALP PSTSINEGSP LWTSIGASTG SPLPEPTTYQ EVSIKMSSVP QETPHATSHP AVPITANSLG SHTVTGGTIT TNSPETSSRT SGAPVTTAAS SLETSRGTSG PPLTMATVSL ETSKGTSGPP VTMATDSLET STGTTGPPVT MTTGSLEPSS GASGPQVSSV KLSTMMSPTT STNASTVPFR NPDENSR

      • Predicted molecular weight

        26 kDa including tags

      • Amino acids

        20 to 253

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab171717 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • CD 43
      • CD43
      • CD43 antigen
      • Galactoglycoprotein
      • GALGP
      • GPL 115
      • GPL115
      • Human gene for sialophorin
      • Leucocyte sialoglycoprotein
      • LEUK_HUMAN
      • Leukocyte large sialoglycoprotein
      • Leukocyte sialoglycoprotein
      • Leukosialin
      • LSN
      • Ly-48
      • Sialophorin
      • sialophorin (gpL115, leukosialin, CD43)
      • Spn
      see all

    • Function

      One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.

    • Tissue specificity

      Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

    • Post-translational
      modifications

      Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.

    • Cellular localization

      Membrane.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CD43 protein (ab171717)
      SDS-PAGE - Recombinant Human CD43 protein (ab171717)
      15% SDS-PAGE analysis of ab171717 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab171717? Please let us know so that we can cite the reference in this datasheet.

    ab171717 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab171717.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com