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Immunology Cell Type Markers CD Non-lineage
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Recombinant Human CD46 protein (BSA and azide free) (ab174047)

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SDS-PAGE - Recombinant Human CD46 protein (BSA and azide free) (ab174047)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human CD46 protein (BSA and azide free)
      See all CD46 proteins and peptides
    • Purity

      > 90 % SDS-PAGE.

    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      HEK 293 cells
    • Accession

      P15529-3
    • Protein length

      Protein fragment
    • Animal free

      No
    • Carrier free

      Yes
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNH TWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLI GEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYL DAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVE NGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCL KVSTSSTTKSPASSASGPRPTYKPPVSNYPGYPKPEEGILDSLD
      • Predicted molecular weight

        34 kDa including tags
      • Amino acids

        35 to 328
      • Tags

        His tag C-Terminus
      • Additional sequence information

        (AAH30594.1)

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab174047 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      Lyophilized from 0.22 μm filtered solution.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 95% PBS, 5% Trehalose

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

    General Info

    • Alternative names

      • AHUS2
      • Antigen defined by monoclonal antibody TRA 2 10
      • Antigen identified by monoclonal antibody TRA 2 10
      • CD46
      • CD46 antigen
      • CD46 antigen complement regulatory protein
      • CD46 molecule
      • CD46 molecule complement regulatory protein
      • Complement membrane cofactor protein
      • MCP
      • MCP_HUMAN
      • Measles virus receptor
      • Membrane cofactor protein
      • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
      • MGC26544
      • MIC10
      • TLX
      • TRA2.10
      • Trophoblast leucocyte common antigen
      • Trophoblast leukocyte common antigen
      • Trophoblast lymphocyte cross reactive antigen
      see all
    • Function

      Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
    • Tissue specificity

      Expressed by all cells except erythrocytes.
    • Involvement in disease

      Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) [MIM:612922]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations.
    • Sequence similarities

      Contains 4 Sushi (CCP/SCR) domains.
    • Domain

      Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.
    • Post-translational
      modifications

      N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
      Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
      In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by Lck.
    • Cellular localization

      Cytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
    • Target information above from: UniProt accession P15529 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CD46 protein (BSA and azide free) (ab174047)
      SDS-PAGE - Recombinant Human CD46 protein (BSA and azide free) (ab174047)

      SDS-PAGE of reduced ab174047 stained overnight with Coomassie Blue. DTT-reduced protein migrates as 45-60 kDa due to glycosylation.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab174047? Please let us know so that we can cite the reference in this datasheet.

    ab174047 has not yet been referenced specifically in any publications.

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