Recombinant human CD47 protein (Fc Chimera Active) (ab220559)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, Flow Cyt, ELISA
Description
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Product name
Recombinant human CD47 protein (Fc Chimera Active)
See all CD47 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human SIRP alpha protein (ab174006) at 2 μg/mL (100 μL/well) can bind Recombinant human CD47 protein (ab220559) with a linear range of 1-31 ng/mL.
Measured by its binding ability in FACS. The binding of Recombinant human CD47 protein (ab220559) to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of ab220559 used is 10 μg/mL. IC50=8.39 μg/mL.
Measured by its binding ability in FACS. Human CD47, Mouse IgG2a Fc Tag, low endotoxin (ab220559) can bind to ACHN cell expressing human SIRP-a. The concentration of CD47 used is 10 μg/mL.
Measured by its binding ability in a BLI assay. Loaded Recombinant human CD47 protein (ab220559) on Protein A Biosensor, can bind Recombinant Human SIRP alpha protein (ab174006) with an affinity constant of 1.1 μM.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QL LFNKTKSVEF TFCNDTVVIP CFVTNMEAQN TTEVYVKWKF KGRDIYTFDG ALNKSTVPTD FSSAKIEVSQ LLKGDASLKM DKSDAVSHTG NYTCEVTELT REGETIIELK YRVVSWFSP -
Predicted molecular weight
41 kDa including tags -
Amino acids
19 to 139 -
Tags
Fc tag C-Terminus -
Additional sequence information
This protein carries a mouse IgG2a Fc tag ((Glu 98-Lys 330) P01863) at the C-terminus (NP_942088).
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Specifications
Our Abpromise guarantee covers the use of ab220559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Flow Cytometry
ELISA
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- Antigen identified by monoclonal antibody 1D8
- Antigenic surface determinant protein OA3
- CD 47
see all -
Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. -
Tissue specificity
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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Immobilized Recombinant Human SIRP alpha protein (ab174006) at 2 μg/mL (100 μL/well) can bind Recombinant human CD47 protein (ab220559) with a linear range of 1-31 ng/mL.
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FACS analysis shows that the binding of Recombinant human CD47 protein (ab220559) to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of ab220559 used is 10 μg/mL. IC50=8.39 μg/mL.
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SDS-PAGE of reduced ab220559 stained overnight with Coomassie Blue. The protein migrates as 55-66 kDa due to glycosylation.
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FACS assay shows that Recombinant human CD47 protein (ab220559) can bind to ACHN cell expressing human SIRP-a. The concentration of ab220559 used is 10 μg/mL.
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Loaded Recombinant human CD47 protein (ab220559) on Protein A Biosensor, can bind Recombinant Human SIRP alpha protein (ab174006) with an affinity constant of 1.1 μM as determined in BLI assay.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab220559 has not yet been referenced specifically in any publications.