Recombinant human CD48 protein (ab179981)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: = 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human CD48 protein
See all CD48 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Human CD244 protein Fc Chimera at 5 μg/ml (100μl/well) can bind Human CD48 with a linear range of 0.1- 4 μg/ml.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
= 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QGHLVHMTVVSGSNVTLNISESLPENYKQLTWFYTFDQKIVEWDSRKSKY FESKFKGRVR LDPQSGALYISKVQKEDNSTYIMRVLKKTGNEQEWKIK LQVLDPVPKPVIKIEKIEDMDD NCYLKLSCVIPGESVNYTWYGDKRPF PKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKN GTVCLSPPCTLARS -
Predicted molecular weight
23 kDa including tags -
Amino acids
27 to 220 -
Tags
His tag C-Terminus -
Additional sequence information
Mature form.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab179981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- Antigen CD48
- B cell membrane protein
- B lymphocyte activation marker BLAST 1
see all -
Function
Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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Human CD48, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Immobilized Human 2B4, Fc Tag at 5 µg/mL (100 µL/well) can bind Human CD48, His Tag with a linear range of 0.156-2.5 µg/mL.
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SDS-PAGE analysis of ab179981 (DTT-reduced) staining overnight with Coomassie Blue. DTT-reduced protein migrates as 38-45 kDa due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab179981 has not yet been referenced specifically in any publications.