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Recombinant Human CD62L protein (Fc Chimera) (ab83824)

  • Datasheet
  • SDS
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Functional Studies - Recombinant Human CD62L protein (Fc Chimera) (ab83824)
  • SDS-PAGE - Recombinant Human CD62L protein (Fc Chimera) (ab83824)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human CD62L protein (Fc Chimera)
    See all CD62L proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    HEK 293 cells
  • Accession

    P14151
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      Theoretical Sequence: WTYHYSEKPMNWQRARRFCRDNYTDLVAIQNKAEIEYLEKTLPFSRSY YWIGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKEDCVEIYIKRN KDAGKWNDDACHKLKAALCYTASCQPWSCSGHGECVEIINNYTCNCDVGY YGPQCQFVIQCEPLEAPELGTMDCTHPLGNFSFSSQCAFSCSEGTNLTGI EETTCGPFGNWSSPEPTCQVIQCEPLSAPDLGIMNCSHPLASFSFTSACT FICSEGTELIGKKKTICESSGIWSNPSPICQKLDKSFSMIKEGDWIPKVD KKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV VVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQD WLNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQ VSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTV DKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • Amino acids

      1 to 343
    • Additional sequence information

      Fused with the Fc region of Human IgG1 at the C-terminus.

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab83824 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C.

      Constituents: PBS, 10% Trehalose, 1% Human serum albumin

    • Reconstitution
      It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

    General Info

    • Alternative names

      • A.11
      • AI528707
      • CD62 antigen ligand
      • CD62 antigen-like family member L
      • CD62L
      • gp90-MEL
      • IgA nephropathy, susceptibility to, included
      • L Selectin
      • L-selectin
      • LAM-1
      • LAM1
      • LECAM1
      • LEU8
      • Leukocyte adhesion molecule 1
      • Leukocyte surface antigen Leu-8
      • Leukocyte-endothelial cell adhesion molecule 1
      • Lnhr
      • LSEL
      • Ly-22
      • Ly-m22
      • Lyam-1
      • LYAM1
      • LYAM1_HUMAN
      • Lymph node homing receptor
      • Lymphocyte adhesion molecule 1
      • Lymphocyte antigen 22
      • Lymphocyte surface MEL-14 antigen
      • MEL-14
      • Pln homing receptor
      • PLNHR
      • Selectin L
      • Selectin, lymphocyte
      • SELL
      • TQ1
      see all
    • Function

      Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
    • Tissue specificity

      Expressed in B cell lines and T lymphocytes.
    • Sequence similarities

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 2 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P14151 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Functional Studies - Recombinant Human CD62L protein (Fc Chimera) (ab83824)
      Functional Studies - Recombinant Human CD62L protein (Fc Chimera) (ab83824)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - Recombinant Human CD62L protein (Fc Chimera) (ab83824)
      SDS-PAGE - Recombinant Human CD62L protein (Fc Chimera) (ab83824)
      1D SDS-PAGE of ab83824 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab83824
      Lane 2: ab83824 treated with PNGase F to remove potential N-linked glycans
      10 μg protein loaded per lane; Deep Purple™ stained.
      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab83824? Please let us know so that we can cite the reference in this datasheet.

    ab83824 has not yet been referenced specifically in any publications.

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