Recombinant human CD70 protein (ab129130)

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Key features and details

  • Expression system: CHO cells
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, HPLC

Description

  • Product name

    Recombinant human CD70 protein
    See all CD70 proteins and peptides

  • Biological activity

    Determined by its ability to stimulate Human IL8 production by Human PBMC using a concentration range of 10.0-25.0 ng/ml. Note: Results may vary with PBMC donors.

  • Purity

    > 95 % SDS-PAGE.
    Purity: > 95%, by SDS-PAGE and HPLC analysis.

  • Expression system

    CHO cells

  • Accession

    P32970

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      19 kDa including tags

    • Amino acids

      39 to 193

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab129130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Form

    Lyophilized

  • Additional notes

    Endotoxin level: < 0.1 ng/µg of soluble CD70. Determined by its ability to stimulate Human IL8 production by Human PBMC using a concentration range of 10.0-25.0 ng/ml. Note: Results may vary with PBMC donors.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstituted ab129130 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

General Info

  • Alternative names

    • CD 27L
    • CD 70
    • CD27 L
    • CD27 LG
    • CD27 ligand
    • CD27-L
    • CD27L
    • CD27LG
    • CD70
    • CD70 antigen
    • CD70 molecule
    • CD70_HUMAN
    • Ki 24 antigen
    • Ki24 antigen
    • Surface antigen CD70
    • TNFSF 7
    • TNFSF7
    • Tumor necrosis factor (ligand) superfamily, member 7
    • Tumor necrosis factor ligand superfamily member 7
    see all

  • Function

    Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Cellular localization

    Membrane.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab129130? Please let us know so that we can cite the reference in this datasheet.

ab129130 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Is the protein provided as a monomer?

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Answer

Here is the amino acid sequence of the CD70/CD27L protein ab129130. Please note that this monomer is the extracellular region, with a His tag at the N terminus.

HHHHHHHHPSPGGSGGQRFAQAQQQLPLESLGWDVAELQLNHTGPQQDPR LYWQGGPALGRSFLHGPELDKGQLRIHRDGIYMVHIQVTLAICSSTTASR HHPTTLAVGICSPASRSISLLRLSFHQGCTIASQRLTPLARGDTLCTNLT GTLLPSRNTDETFFGVQWVRP

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