Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIW PEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEV TLSVKADFPTPSISDFEIPT SNIRRIICSTSGGFPEPHLSWLENGEEL NAINTTVSQDPETELYAVSSKLDFNMTTNHSF MCLIKYGHLRVNQTFN WNTTKQEHFPDN
    • Molecular weight
      50 kDa including tags
    • Amino acids
      35 to 242
    • Additional sequence information
      (NP_005182.1) ab173993 is fused with Fc fragment of Human IgG1 at the C terminus.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab173993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its ability to induce IL2 secretion by Jurkat Human acute T cell leukemia cells. The ED50 for this effect is typically 0.025 - 0.15 µg/mL in the presence of PHA.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Additional notes

    After reconstitution store under sterile conditions for 1 month at 4°C - 8°C or 3 months at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Please see notes section.

    Information available upon request.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in 100 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • Activation B7-1 antigen
    • B lymphocyte activation antigen B7
    • B7
    • B7-1
    • B7-1 antigen
    • B7.1
    • BB1
    • CD28 antigen ligand 1
    • CD28LG
    • CD28LG1
    • CD80
    • CD80 antigen
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
    • CD80 molecule
    • CD80_HUMAN
    • Costimulatory factor CD80
    • costimulatory molecule variant IgV-CD80
    • CTLA-4 counter-receptor B7.1
    • LAB7
    • T-lymphocyte activation antigen CD80
    see all
  • Function
    Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificity
    Expressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • Reduced SDS-PAGE analysis of ab173993, stained overnight with Coomassie Blue.

    Predicted MW of protein fragment with is C-Fc Tag 50 kDa, but DTT-reduced protein migrates as 70-90 kDa due to glycosylation.

References

ab173993 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab173993.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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