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    recombinant-human-cd97-protein-ab131865.pdf

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Recombinant Human CD97 protein (ab131865)

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SDS-PAGE - Recombinant Human CD97 protein (ab131865)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, WB, SDS-PAGE

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    Description

    • Product name

      Recombinant Human CD97 protein
    • Expression system

      Wheat germ
    • Accession

      P48960-2
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGGRVFLAFCVWLTLPGAETQDSRGCARWCPQNSSCVNATACRCNPGFSS FSEIITTPTETCDDINECATPSKVSCGKFSDCWNTEGSYDCVCSPGYEPV SGAKTFKNESENTCQDVDECSSGRHQCDSSTVCFNTVGSYSCRCRPGWKP RHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRFFDKVQDLGRDSKTSS AEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNLEDIMRILAK SLPKGPFTYISPSNTELTLMIQERGDKNVTMGQSSARMKLNWAVAAGAED PGPAVAGILSIQNMTTLLANASLNLHSKKQAELEEIYESSIRGVQLRRLS AVNSIFLSHNNTKELNSPILFAFSHLESSDGEAGRDPPAKDVMPGPRQEL LCAFWKSDSDRGGHWATEGCQVLGSKNGSTTCQCSHLSSFAILMAHYDVE DWKLTLITRVGLALSLFCLLLCILTFLLVRPIQGSRTTIHLHLCICLFVG STIFLAGIENEGGQVGLRCRLVAGLLHYCFLAAFCWMSLEGLELYFLVVR VFQGQGLSTRWLCLIGYGVPLLIVGVSAAIYSKGYGRPRYCWLDFEQGFL WSFLGPVTFIILCNAVIFVTTVWKLTQKFSEINPDMKKLKKARALTITAI AQLFLLGCTWVFGLFIFDDRSLVLTYVFTILNCLQGAFLYLLHCLLNKKV REEYRKWACLVAGGSKYSEFTSTTSGTGHNQTRALRASESGI
      • Predicted molecular weight

        107 kDa including tags
      • Amino acids

        1 to 742

    Specifications

    Our Abpromise guarantee covers the use of ab131865 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Western blot

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • ADGRE5
      • Adhesion G protein-coupled receptor E5
      • CD 97
      • CD_antigen=CD97
      • CD97
      • CD97 antigen
      • CD97 antigen subunit beta
      • CD97 molecule
      • CD97_HUMAN
      • Leukocyte antigen CD97
      • Seven span transmembrane protein
      • Seven transmembrane helix receptor
      • Seven transmembrane heterodimeric receptor associated with inflammation
      • TM7LN 1
      • TM7LN1
      see all
    • Function

      Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
    • Tissue specificity

      Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
      Contains 5 EGF-like domains.
      Contains 1 GPS domain.
    • Domain

      The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
      Binding to chondroitin sulfate is mediated by the fourth EGF domain.
    • Post-translational
      modifications

      Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
    • Cellular localization

      Cell membrane and Secreted > extracellular space.
    • Target information above from: UniProt accession P48960 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CD97 protein (ab131865)
      SDS-PAGE - Recombinant Human CD97 protein (ab131865)
      12.5% SDS-PAGE analysis of ab131865 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab131865? Please let us know so that we can cite the reference in this datasheet.

    ab131865 has not yet been referenced specifically in any publications.

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