Description

  • Product name

    Recombinant human Cdc25A protein
    See all Cdc25A proteins and peptides
  • Biological activity

    Specific activity 31 nmol phosphate released/min/mg.
  • Purity

    > 80 % Densitometry.
    ab105905 was determined to be >80% pure by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      94 kDa including tags
    • Amino acids

      1 to 524

Specifications

Our Abpromise guarantee covers the use of ab105905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Cdc 25a
    • CDC25A
    • CDC25A2
    • CDC25A2 CAG isoform
    • Cell division cycle 25 homolog A (S. pombe)
    • Cell division cycle 25A
    • Cell division cycle 25A isoform a
    • Cell division cycle 25A isoform b
    • D9Ertd393e
    • Dual specificity phosphatase Cdc25A
    • M phase inducer phosphatase 1
    • M-phase inducer phosphatase 1
    • MGC115549
    • MPIP1_HUMAN
    see all
  • Function

    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
  • Sequence similarities

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Domain

    The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
  • Post-translational
    modifications

    Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
  • Information by UniProt

Images

  • The specific activity of Cdc25A (ab105905) was determined to be 36 phosphate released/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab105905
  • SDS PAGE analysis of ab105905
  • The specific activity of ab105905 was determined to be 31 nmol phosphate released/min/mg.
  • The purity of ab105905 was determined to be >80% by densitometry.

References

ab105905 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products.

ab104897 is a recombinant full length Human Cdc25A (amino acids 1-524) with N terminal His and DDDDK tags; Since it is tagged the actual size is higher than the predicted MWt (63.2kDa, 560 amino acids inclusive of tags).

The other product (ab105905) is also tagged as the datasheet indicates ie. full-length recombinant Human Cdc25A (amino acids 1-524) with a N terminal proprietary tag. MWt 94 kDa.

If you need any further assistance in the future, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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