Product nameRecombinant human CDC42 binding protein kinase alpha
Biological activitySpecific activity: 101nmol/min/mg.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Amino acids1 to 473
Our Abpromise guarantee covers the use of ab59979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CDC42 binding protein kinase alpha
- CDC42 binding protein kinase alpha (DMPK like)
FunctionMay act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
Tissue specificityAbundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 CNH domain.
Contains 1 CRIB domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab59979 has not yet been referenced specifically in any publications.