Recombinant Human CDC5L protein (ab132350)
Key features and details
- Expression system: Wheat germ
- Suitable for: WB, SDS-PAGE, ELISA
Description
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Product name
Recombinant Human CDC5L protein -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARW YEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHY EFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELE MLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQK KRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGE LRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAP QISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVAL RTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQ VVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNI NPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEK ELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSE VNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKG KTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHG ELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINR GHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELR TFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKS KF -
Predicted molecular weight
119 kDa including tags -
Amino acids
1 to 802
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab132350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
ELISA
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- CDC5
- CDC5 cell division cycle 5-like (S. pombe)
- Cdc5 related protein
see all -
Function
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. -
Tissue specificity
Ubiquitously expressed in both fetal and adult tissues. -
Involvement in disease
A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2. -
Sequence similarities
Belongs to the CEF1 family.
Contains 2 HTH myb-type DNA-binding domains. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8. -
Cellular localization
Nucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab132350 has not yet been referenced specifically in any publications.