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Cell Biology Cell Cycle Kinases/Phosphatases Cdks
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Recombinant human CDK1 + Cyclin-A1 protein (ab104617)

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Functional Studies - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
  • SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
  • SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
  • Functional Studies - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
  • SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 75% Densitometry
  • Active: Yes
  • Suitable for: Functional Studies, WB, SDS-PAGE

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Description

  • Product name

    Recombinant human CDK1 + Cyclin-A1 protein
  • Biological activity

    The Specific activity of ab104617 was determined to be 38 nmol/min/mg.
  • Purity

    > 75 % Densitometry.
    Purity was determined to be >75% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Associated products

  • Related Products

    • Anti-Cyclin A1 antibody (ab53699)

Specifications

Our Abpromise guarantee covers the use of ab104617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CCNA1
    • CDC2
    • CDC28A
    • CDK1
    • CDK1_HUMAN
    • Cell cycle controller CDC2
    • Cell division control protein 2 homolog
    • Cell division cycle 2 G1 to S and G2 to M
    • Cell division protein kinase 1
    • Cyclin A1
    • Cyclin-dependent kinase 1
    • DKFZp686L20222
    • MGC111195
    • p34 protein kinase
    • P34CDC2
    see all
  • Function

    Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis (PubMed:26549230).
  • Tissue specificity

    Isoform 2 is found in breast cancer tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation at Thr-161 by CAK/CDK7 activates kinase activity. Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylation by PKMYT1 and WEE1 takes place during mitosis to keep CDK1-cyclin-B complexes inactive until the end of G2. By the end of G2, PKMYT1 and WEE1 are inactivated, but CDC25A and CDC25B are activated. Dephosphorylation by active CDC25A and CDC25B at Thr-14 and Tyr-15, leads to CDK1 activation at the G2-M transition. Phosphorylation at Tyr-15 by WEE2 during oogenesis is required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, leading to prevent meiotic reentry. Phosphorylation by WEE2 is also required for metaphase II exit during egg activation to ensure exit from meiosis in oocytes and promote pronuclear formation. Phosphorylated at Tyr-4 by PKR/EIF2AK2 upon genotoxic stress. This phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest. In response to UV irradiation, phosphorylation at Tyr-15 by PRKCD activates the G2/M DNA damage checkpoint.
    Polyubiquitinated upon genotoxic stress.
  • Cellular localization

    Nucleus. Cytoplasm. Mitochondrion. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasmic during the interphase. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Target information above from: UniProt accession P06493 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    Functional Studies - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    The specific activity of CDK1 + Cyclin-A1 (ab104617) was determined to be 48 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    SDS PAGE analysis of ab104617
  • SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    SDS PAGE analysis of ab104617
  • Functional Studies - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    Functional Studies - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    Kinase Assay demonstrating specific activity of ab104617.
  • SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    SDS-PAGE - Recombinant human CDK1 + Cyclin-A1 protein (ab104617)
    SDS-PAGE showing ab104617 at approximately 58 and 81kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab104617? Please let us know so that we can cite the reference in this datasheet.

    ab104617 has not yet been referenced specifically in any publications.

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