Description

  • Product name

    Recombinant human CDK1 + Cyclin A2 protein
  • Biological activity

    Specific activity: 178 nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab64299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CCN1
    • CCNA
    • CCNA2
    • CDC2
    • CDC28A
    • CDK1
    • Cyclin A
    • Cyclin A2
    • Cyclin dependent kinase 1
    • p34 protein kinase
    see all
  • Relevance

    Cyclin A2 is essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Cellular localization

    Cytoplasmic and Nuclear

Images

  • The specific activity of CDK1 + Cyclin A2 (ab64299) was determined to be 205 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab64299
  • SDS PAGE analysis of ab64299
  • Sample Kinase Activity Plot.

  • SDS page analysis of ab64299.

References

ab64299 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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