• Product name

    Recombinant human CDK2 + CCNE1 protein (ab85639)
    See all CDK2 + CCNE1 proteins and peptides
  • Biological activity

    The Specific activity of ab85639 was determined to be 404 nmol/min/mg.
  • Purity

    > 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

  • Nature

    • Species

  • Description

    Recombinant human CDK2 + CCNE1 protein


Our Abpromise guarantee covers the use of ab85639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies


  • Form

  • Additional notes

    ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CCNE
    • CCNE 1
    • CCNE1
    • Cdc2 related protein kinase
    • Cdk 2
    • CDK2
    • Cell division kinase 2
    • Cell division protein kinase 2
    • Cyclin dependent kinase 2
    • Cyclin E1
    • Cyclin Es
    • Cyclin Et
    • CyclinE
    • G1/S specific cyclin E
    • G1/S specific cyclin E1
    • p33 protein kinase
    • p33(CDK2)
    see all
  • Relevance

    CDK2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. CCNE1 (Cyclin E) is a regulatory subunit of CDK2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
  • Cellular localization

    CCNE1: Nuclear.


  • The specific activity of CDK2 + CCNE1 (ab85639) was determined to be 345 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab85639
  • SDS PAGE analysis of ab85639
  • The Specific activity of ab85639 was determined to be 404 nmol/min/mg.
  • SDS-PAGE showing ab85639 at approximately 58kDa (CDK2) and 73kDa (CCNE1).


This product has been referenced in:

  • Wang Y  et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). Read more (PubMed: 29853338) »
  • Kitagawa K  et al. Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development. Mol Cell Biol 34:2732-44 (2014). Read more (PubMed: 24820417) »
See all 2 Publications for this product

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