This product is an active protein and may elicit a biological response in vivo, handle with caution.
Cdc2 related protein kinase
Cell division kinase 2
Cell division protein kinase 2
Cyclin dependent kinase 2
G1/S specific cyclin E
G1/S specific cyclin E1
p33 protein kinase
CDK2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
CCNE1 (Cyclin E) is a regulatory subunit of CDK2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Functional Studies - CDK2 + CCNE1 protein (Active) (ab85639)
The Specific activity of ab85639 was determined to be 404 nmol/min/mg.
SDS-PAGE - CDK2 + CCNE1 protein (Active) (ab85639)
SDS-PAGE showing ab85639 at approximately 58kDa (CDK2) and 73kDa (CCNE1).
This product has been referenced in:
Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem26:3491-3501 (2018).
Read more (PubMed: 29853338) »
Kitagawa K et al. Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development. Mol Cell Biol34:2732-44 (2014).
Read more (PubMed: 24820417) »