Recombinant human CDK2 + CCNE1 protein (ab85639) (ab85639)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CDK2 + CCNE1 protein (ab85639) -
Biological activity
The Specific activity of ab85639 was determined to be 404 nmol/min/mg.
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Purity
> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Tags
GST tag N-Terminus
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Amino Acid Sequence 2
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Species
Human -
Tags
GST tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab85639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Cellular localization
Cyclin E1: Nucleus.
Images
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The specific activity of CDK2 + CCNE1 (ab85639) was determined to be 345 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab85639
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SDS PAGE analysis of ab85639
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The Specific activity of ab85639 was determined to be 404 nmol/min/mg.
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SDS-PAGE showing ab85639 at approximately 58kDa (CDK2) and 73kDa (CCNE1).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab85639 has been referenced in 3 publications.
- Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). PubMed: 29853338
- Azzarelli R et al. Multi-site Neurogenin3 Phosphorylation Controls Pancreatic Endocrine Differentiation. Dev Cell 41:274-286.e5 (2017). PubMed: 28457793
- Kitagawa K et al. Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development. Mol Cell Biol 34:2732-44 (2014). PubMed: 24820417