Recombinant human CDK2 + Cyclin A2 protein (ab196060)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 64% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, His tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CDK2 + Cyclin A2 protein -
Biological activity
Specific activity 390 pmol/min/µg.
Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/ml) substrate and 20 µM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase reagents
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Purity
>= 64 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
ENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIRE ISLLKELNH PNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGI PLPLIKSYLFQLLQGLAFCHS HRVLHRDLKPQNLLINTEGAIKLADFG LARAFGVPVRTYTHEVVTLWYRAPEILLGCKYY STAVDIWSLGCIFAE MVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSF PKW ARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPV PHLRL -
Predicted molecular weight
61 kDa including tags -
Amino acids
2 to 298 -
Tags
GST tag N-Terminus -
Additional sequence information
(NM_001798)
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Amino Acid Sequence 2
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Species
Human -
Sequence
EVPDYHE DIHTYLREME VKCKPKVGYM KKQPDITNSM RAILVDWLVE VGEEYKLQNE TLHLAVNYID RFLSSMSVLR GKLQLVGTAA MLLASKFEEI YPPEVAEFVY ITDDTYTKKQ VLRMEHLVLK VLTFDLAAPT VNQFLTQYFL HQQPANCKVE SLAMFLGELS LIDADPYLKY LPSVIAGAAF HLALYTVTGQ SWPESLIRKT GYTLESLKPC LMDLHQTYLK APQHAQQSIR EKYKNSKYHG VSLLNPPETL NL -
Predicted molecular weight
34 kDa including tags -
Amino acids
174 to 432 -
Tags
His tag N-Terminus , GST tag N-Terminus -
Additional sequence information
Cyclin A2 (NM_001237)
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab196060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.004% EGTA, 0.003% EDTA, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CCN1
- CCNA
- Cell division protein kinase 2
see all -
Relevance
CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. -
Cellular localization
Nuclear
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196060 has not yet been referenced specifically in any publications.