Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence 1
    • Accession
    • Species
      Human
    • Sequence
      MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIR EISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIP LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLAR AFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRR ALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSK VVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
    • Molecular weight
      34 kDa including tags
    • Amino acids
      1 to 298
    • Additional sequence information
      Gene accession number is NM_001798.
    Amino Acid Sequence 2
    • Accession
    • Species
      Human
    • Molecular weight
      57 kDa including tags
    • Amino acids
      174 to 432
    • Tags
      GST tag N-Terminus
    • Additional sequence information
      Gene accession number NM_001237

Associated products

Specifications

Our Abpromise guarantee covers the use of ab198143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: 203 pmol/min/µg.

     

    Assay Conditions: Assay was done in a Kinase buffer using Histone H1 ( 0.1 mg/ml at final concentration) as a substrate with 20 μM ATP at 30°C for 45 min. The amount of ATP transferred was calculated.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    > 98 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    The two proteins are co-experessed in a baculovirus Sf9 insect cell expression
    system.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 10% Glycerol, 2.32% Sodium chloride, 0.61% Glutathione, 0.03% DTT, 0.33% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • CCN1
    • CCNA
    • Cell division protein kinase 2
    • Cyclin A
    • Cyclin A2
    • Cyclin dependent kinase 2
    • p33 protein kinase
    see all
  • Relevance
    CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
  • Cellular localization
    Nuclear

Images

  • Activity assay using ab198143.

  • 4-20% SDS-PAGE Coomassie staining. Lane 1: 3.48 µg ab198143. Lane 2: Protein Marker

References

ab198143 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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