Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human CDK2 + Cyclin A2 protein
See all CDK2 + Cyclin A2 proteins and peptides
Biological activitySpecific activity determined to be 915 nmol/min/mg.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab51407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Cell division protein kinase 2
RelevanceCDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
The specific activity of CDK2 + Cyclin A2 (ab51407) was determined to be 778 nmol/min/mg as per activity assay protocol
SDS PAGE analysis of ab51407
Kinase activity assay. Specific activity 915 nmol/min/mg.
Purity determined to be > 70% by densitometry.
CDK2 molecular weight 58 kDa
Cyclin A2 molecular weight 78 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51407 has been referenced in 1 publication.
- Kitagawa K et al. Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development. Mol Cell Biol 34:2732-44 (2014). PubMed: 24820417