Description

  • Product name

    Recombinant human CDK3 + CCNE1 protein
  • Biological activity

    The Specific activity of ab85643 was determined to be 20 nmol/min/mg.
  • Purity

    > 90 % SDS-PAGE.
    Purity was determined to be >90% by densitometry.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab85643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CCNE
    • CCNE 1
    • CCNE1
    • Cdk 3
    • Cell division kinase 3
    • Cell division protein kinase 3
    • Cyclin dependent kinase 3
    • Cyclin E1
    • Cyclin Es
    • Cyclin Et
    • CyclinE
    • G1/S specific cyclin E
    • G1/S specific cyclin E1
    • OTTHUMP00000206828
    • p36
    see all
  • Relevance

    CDK3 is a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. CCNE1 (Cyclin E) is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
  • Cellular localization

    CCNE1: Nuclear

Images

  • The specific activity of CDK3 + CCNE1 (ab85643) was determined to be 23 nmol/min/mg as per activity assay protocol
  • SDS PAGE analysis of ab85643
  • SDS PAGE analysis of ab85643
  • The Specific activity of ab85643 was determined to be 20 nmol/min/mg.
  • SDS-PAGE showing ab85643 at approximately 60kDa (CDK3) and 73kDa (CCNE1).

References

ab85643 has not yet been referenced specifically in any publications.

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