Product nameRecombinant Human Cdk4 + Cyclin D1 protein
Protein lengthFull length protein
SourceBaculovirus infected Sf9 cells
Amino Acid Sequence
Our Abpromise guarantee covers the use of ab55695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activity14nmol/min/mg using protein substrate Rb (773-928).
Additional notes14nmol/min/mg using protein substrate Rb (773-928).
ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride
- B cell CLL lymphoma 1
- BCL 1 oncogene
RelevanceCDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase. Phosphorylation at Thr-172 is necessary for enzymatic activity. CDK4 mutations are involved in tumor formation, with defects in CDK4 resulting in cutaneous malignant melanoma 3 (CMM3). Cyclin D1 is essential for the control of the cell cycle at the G1/S transition. It interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
This product has been referenced in:
- Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). Read more (PubMed: 29853338) »
- Wang H et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). Read more (PubMed: 28607489) »