CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase. Phosphorylation at Thr-172 is necessary for enzymatic activity. CDK4 mutations are involved in tumor formation, with defects in CDK4 resulting in cutaneous malignant melanoma 3 (CMM3).
Cyclin D1 is essential for the control of the cell cycle at the G1/S transition. It interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
Functional Studies - Cdk4 + Cyclin D1 protein (ab55695)
Kinase assay using ab55695 using protein substrate Rb. The specific activity of CDK4/CyclinD1 is 14 nmol/min/mg.
SDS-PAGE - Cdk4 + Cyclin D1 protein (Tagged) (ab55695)
SDS-PAGE analysis of ab55695 with molecular weight markers. Approximate molecular weights: 57kDa (CDK4)/61kDa (CyclinD1).
This product has been referenced in:
Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem26:3491-3501 (2018).
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