Overview

  • Product name
    Recombinant Human Cdk4 + Cyclin D1 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab55695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    14nmol/min/mg using protein substrate Rb (773-928).
  • Applications

    SDS-PAGE

    Functional Studies

  • Form
    Liquid
  • Additional notes
    14nmol/min/mg using protein substrate Rb (773-928).

    ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

General Info

  • Alternative names
    • B cell CLL lymphoma 1
    • BCL 1 oncogene
    • BCL1
    • Cell division protein kinase 4
    • CMM3
    • Cyclin dependent kinase 4
    • PRAD1
    • PSK J3
    see all
  • Relevance
    CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase. Phosphorylation at Thr-172 is necessary for enzymatic activity. CDK4 mutations are involved in tumor formation, with defects in CDK4 resulting in cutaneous malignant melanoma 3 (CMM3). Cyclin D1 is essential for the control of the cell cycle at the G1/S transition. It interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
  • Cellular localization
    Nuclear

Images

  • Kinase assay using ab55695 using protein substrate Rb. The specific activity of CDK4/CyclinD1 is 14 nmol/min/mg.
  • SDS-PAGE analysis of ab55695 with molecular weight markers. Approximate molecular weights: 57kDa (CDK4)/61kDa (CyclinD1).

References

This product has been referenced in:
  • Wang Y  et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). Read more (PubMed: 29853338) »
  • Wang H  et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). Read more (PubMed: 28607489) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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