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    recombinant-human-cdk4-protein-ab126909.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Cdks
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Recombinant Human Cdk4 protein (ab126909)

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SDS-PAGE - Recombinant Human Cdk4 protein (ab126909)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 95% Densitometry
    • Tags: GST tag N-Terminus
    • Suitable for: SDS-PAGE, WB

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    Description

    • Product name

      Recombinant Human Cdk4 protein
      See all Cdk4 proteins and peptides
    • Purity

      > 95 % Densitometry.
      Purity was determined to be >95% by densitometry. Affinity purified.
    • Expression system

      Baculovirus infected Sf9 cells
    • Accession

      P11802
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Predicted molecular weight

        57 kDa including tags
      • Tags

        GST tag N-Terminus

    Associated products

    • Related Products

      • Anti-Cdk4 antibody [EPR4513-54-3] (ab108355)
      • Anti-Cdk4 antibody (ab95255)

    Specifications

    Our Abpromise guarantee covers the use of ab126909 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • Cdk 4
      • cdk4
      • CDK4 protein
      • CDK4_HUMAN
      • Cell division kinase 4
      • Cell division protein kinase 4
      • CMM 3
      • CMM3
      • Crk3
      • Cyclin dependent kinase 4
      • Cyclin-dependent kinase 4
      • Melanoma cutaneous malignant 3
      • MGC14458
      • p34 cdk4
      • PSK J3
      • PSK-J3
      see all
    • Function

      Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
    • Involvement in disease

      Defects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
    • Cellular localization

      Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
    • Target information above from: UniProt accession P11802 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Cdk4 protein (ab126909)
      SDS-PAGE - Recombinant Human Cdk4 protein (ab126909)
      SDS-PAGE using ab126909

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab126909? Please let us know so that we can cite the reference in this datasheet.

    ab126909 has not yet been referenced specifically in any publications.

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