Product nameRecombinant Human Cdk4 protein
See all Cdk4 proteins and peptides
Purity> 90 % SDS-PAGE.
ab169875 was expressed in E.coli as inclusion bodies. The final product was refolded and chromatographically purified.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMASMTGGQQMGRGEFMATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALK SVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDR EIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANC IVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEV LLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPE DDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRIS AFRALQHSYLHKDEGNPE
Predicted molecular weight35 kDa including tags
Amino acids1 to 303
TagsT7 tag N-Terminus
- Anti-Cdk4 antibody [EPR4513-32-7] (ab108357)
- Anti-T7 tag® antibody (DyLight® 550) (ab117595)
- Anti-T7 tag® antibody (Agarose) (ab1230)
- Anti-Cdk4 antibody (ab137675)
- Anti-T7 tag® antibody (HRP) (ab21477)
- Anti-T7 tag® antibody (Phycoerythrin) (ab72563)
- Anti-T7 tag® antibody (SureLight® Allophycocyanin) (ab72565)
- Anti-T7 tag® antibody (ab9115)
- Anti-T7 tag® antibody (ab9138)
- Anti-T7 tag® antibody (ab97964)
Our Abpromise guarantee covers the use of ab169875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituent: 0.32% Tris HCl
Note: Contains NaCl, KCl, EDTA, arginine, DTT and glycerol.
- Cdk 4
- CDK4 protein
FunctionSer/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Involvement in diseaseDefects in CDK4 are a cause of susceptibility to cutaneous malignant melanoma type 3 (CMM3) [MIM:609048]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Cellular localizationCytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
- Information by UniProt
ab169875 has not yet been referenced specifically in any publications.