- Datasheet
- Specific References
- Protocols
Overview
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Product nameRecombinant Human CDK5R1(p25) protein.
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Protein lengthFull length protein
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DescriptionRecombinant Human CDK5R1 protein
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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Accession
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SpeciesHuman
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Molecular weight49 kDa including tags
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Amino acids98 to 307
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Specifications
Our Abpromise guarantee covers the use of ab125653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Purity> 85 % Densitometry.
Purity was determined to be >85% by densitometry. -
FormLiquid
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride
General Info
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Alternative names
- CD5R1_HUMAN
- CDK5 activator 1
- CDK5R1
see all -
Functionp35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
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Tissue specificityBrain and neuron specific.
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Sequence similaritiesBelongs to the cyclin-dependent kinase 5 activator family.
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Post-translational
modificationsThe p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35. -
Cellular localizationCell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
- Information by UniProt
Images
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SDS-PAGE - Recombinant Human CDK5R1(p25) protein. (ab125653)SDS-PAGE analysis of ab125653.
References
ab125653 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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