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    recombinant-human-cdk5r1p25-protein-ab125653.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Cdk Regulators
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Recombinant Human CDK5R1(p25) protein. (ab125653)

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SDS-PAGE - Recombinant Human CDK5R1(p25) protein. (ab125653)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 85% Densitometry
    • Suitable for: WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human CDK5R1(p25) protein.
    • Purity

      > 85 % Densitometry.
      Purity was determined to be >85% by densitometry.
    • Expression system

      Baculovirus infected Sf9 cells
    • Accession

      Q15078
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Predicted molecular weight

        49 kDa including tags
      • Amino acids

        98 to 307
    • Description

      Recombinant Human CDK5R1 protein

    Specifications

    Our Abpromise guarantee covers the use of ab125653 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • CD5R1_HUMAN
      • CDK5 activator 1
      • CDK5R1
      • Cyclin-dependent kinase 5 activator 1
      • Cyclin-dependent kinase 5 regulatory subunit 1
      • p23
      • p25
      • p35
      • Tau protein kinase II 23 kDa subunit
      • TPKII regulatory subunit
      see all
    • Function

      p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
    • Tissue specificity

      Brain and neuron specific.
    • Sequence similarities

      Belongs to the cyclin-dependent kinase 5 activator family.
    • Post-translational
      modifications

      The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
      Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
    • Cellular localization

      Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
    • Target information above from: UniProt accession Q15078 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CDK5R1(p25) protein. (ab125653)
      SDS-PAGE - Recombinant Human CDK5R1(p25) protein. (ab125653)
      SDS-PAGE analysis of ab125653.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab125653? Please let us know so that we can cite the reference in this datasheet.

    ab125653 has not yet been referenced specifically in any publications.

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