Product nameRecombinant human CDK6 + CCND1 protein
Protein lengthFull length protein
SourceBaculovirus infected Sf9 cells
Amino Acid Sequence
Our Abpromise guarantee covers the use of ab84559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific activity of CDK6 + CCND1 was determined to be 9.3 nmol/min/mg as per activity assay protocol.
ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol, 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- B cell CLL/lymphoma 1
- BCL 1 oncogene
RelevanceCDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors (1). The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein (2). CCND1 is essential for the control of the cell cycle at the G1/S (start) transition
ab84559 has not yet been referenced specifically in any publications.