Product nameRecombinant human CDK6 + CCND3 protein
See all CDK6 + CCND3 proteins and peptides
Protein lengthFull length protein
SourceBaculovirus infected Sf9 cells
Amino Acid Sequence
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab84557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activitySpecific activity of ab84557 was determined to be 36 nmol/min/mg.
ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol, 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CCND 3
- CDK 6
RelevanceCDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 are members of a growing superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating the activity of cyclin-dependent kinases (cdks). All three known members of the D-type subfamily form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6.
Cellular localizationCCND3: Cytoplasm. Nucleus
This product has been referenced in:
- Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). Read more (PubMed: 29853338) »