Recombinant human CDK6 + CCND3 protein (ab84557)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human CDK6 + CCND3 protein
See all CDK6 + CCND3 proteins and peptides -
Biological activity
Specific activity of ab84557 was determined to be 36 nmol/min/mg.
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Purity
> 75 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
His tag N-Terminus
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab84557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CCND 3
- CCND3
- CDK 6
see all -
Relevance
CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 are members of a growing superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating the activity of cyclin-dependent kinases (cdks). All three known members of the D-type subfamily form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6. -
Cellular localization
CCND3: Cytoplasm. Nucleus
Images
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The specific activity of CDK6 + CCND3 (ab84557) was determined to be 40 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab84557
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SDS PAGE analysis of ab84557
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SDS-PAGE of ab84557. CDK6 approximate MW 40kDa and CCND3 approximate MW 35kDa.
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Kinase Assay: Specific activity of CDK6/CCND3 was determined to be 36 nmol/min/mg using protein substrate Rb.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab84557 has been referenced in 1 publication.
- Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). PubMed: 29853338