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    recombinant-human-cdk6-ccnd3-protein-ab84557.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Cdks
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Recombinant human CDK6 + CCND3 protein (ab84557)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human CDK6 + CCND3 protein (ab84557)
  • SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
  • SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
  • SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
  • Functional Studies - Recombinant human CDK6 + CCND3 protein (ab84557)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: WB, Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human CDK6 + CCND3 protein
    See all CDK6 + CCND3 proteins and peptides
  • Biological activity

    Specific activity of ab84557 was determined to be 36 nmol/min/mg.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Tags

      His tag N-Terminus

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-Cyclin D3/CCND3 antibody [DCS2.2] (ab28283)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-Cdk6 antibody [8H4] (ab54576)
  • Substrate reagent

    • Recombinant Human Rb protein (ab56270)

Specifications

Our Abpromise guarantee covers the use of ab84557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CCND 3
    • CCND3
    • CDK 6
    • CDK6
    • Cell division protein kinase 6
    • Crk 2
    • Crk2
    • Cyclin D3
    • Cyclin dependent kinase 6
    • CyclinD3
    • D3 type cyclin
    • G1 S specific cyclin D3
    • G1/S specific cyclin D3
    • MGC59692
    • p40
    • PLSTIRE
    • Serine/threonine protein kinase PLSTIRE
    • STQTL11
    see all
  • Relevance

    CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 are members of a growing superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating the activity of cyclin-dependent kinases (cdks). All three known members of the D-type subfamily form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6.
  • Cellular localization

    CCND3: Cytoplasm. Nucleus

Images

  • Functional Studies - Recombinant human CDK6 + CCND3 protein (ab84557)
    Functional Studies - Recombinant human CDK6 + CCND3 protein (ab84557)
    The specific activity of CDK6 + CCND3 (ab84557) was determined to be 40 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
    SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
    SDS PAGE analysis of ab84557
  • SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
    SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
    SDS PAGE analysis of ab84557
  • SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
    SDS-PAGE - Recombinant human CDK6 + CCND3 protein (ab84557)
    SDS-PAGE of ab84557. CDK6 approximate MW 40kDa and CCND3 approximate MW 35kDa.
  • Functional Studies - Recombinant human CDK6 + CCND3 protein (ab84557)
    Functional Studies - Recombinant human CDK6 + CCND3 protein (ab84557)
    Kinase Assay: Specific activity of CDK6/CCND3 was determined to be 36 nmol/min/mg using protein substrate Rb.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab84557? Please let us know so that we can cite the reference in this datasheet.

ab84557 has been referenced in 1 publication.

  • Wang Y  et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). PubMed: 29853338

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