Recombinant human CDK9 + CyclinT2 protein (ab207963)

Overview

  • Product name

    Recombinant human CDK9 + CyclinT2 protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence 1
    • Accession
    • Species

      Human
    • Molecular weight

      68 kDa including tags
    • Amino acids

      1 to 372
    • Tags

      GST tag N-Terminus
    Amino Acid Sequence 2
    • Accession
    • Species

      Human
    • Molecular weight

      105 kDa including tags
    • Amino acids

      1 to 730
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab207963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab207963 was determined to be 6 nmol/min/mg

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity

    > 70 % Densitometry.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.5
    Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • C 2k
    • C-2K
    • CCNT2
    • CDC2
    • CDC2L4
    • Cdk 9
    • Cdk9
    • Cell division cycle 2 like protein kinase 4
    • Cell division protein kinase 9
    • CTK1
    • Cyclin dependent kinase 9
    • Cyclin T2a
    • Cyclin T2b
    • CycT2
    • SDS stable vimentin bound DNA fragment HEF42VIM22
    • Serine threonine protein kinase PITALR
    • Subunit of positive elongation transcription factor b
    see all
  • Relevance

    Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).
  • Cellular localization

    Nuclear

Images

  • Kinase Assay demonstrating specific activity of ab207963

  • SDS-PAGE showing ab207963

References

ab207963 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab207963.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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