This product is an active protein and may elicit a biological response in vivo, handle with caution.
cyclin dependent kinase 9
Cdk9 is a member of the cyclin-dependent kinase pair (CDK9/Cyclin K) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). The cdk9/cyclin k complex also has a kinase activity towards the CTD of RNAP II and can substitute for P-TEFB in vitro. In vitro, it phosphorylates retinoblastoma and myelin basic protein.
Cyclin K (CCNK) belongs to the highly conserved cyclin family and is thought to play a role in transcriptional regulation. CCNK and its kinase partner CDK9 are subunits of the transcription elongation factor pTEFB. CCNK may play a dual role in regulating CDK and RNA polymerase II activities.
SDS-PAGE - Recombinant human CDK9+CyclinK protein (ab70320)
SDS-PAGE showing CDK9/CyclinK at approximaltely 67kDa.
Functional Studies - Recombinant human CDK9+CyclinK protein (ab70320)
Kinase Assay demonstrating CDK9/CyclinK specific activity.
This product has been referenced in:
Mikula M et al. Halogenated imidazole derivatives block RNA polymerase II elongation along mitogen inducible genes. BMC Mol Biol11:4 (2010).
Read more (PubMed: 20078881) »