Recombinant Human CDKL5 protein (ab131695)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Description
-
Product name
Recombinant Human CDKL5 protein -
Purity
> 90 % SDS-PAGE.
Assessed by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
84 kDa including tags -
Amino acids
1 to 498
-
Specifications
Our Abpromise guarantee covers the use of ab131695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
General Info
-
Alternative names
- Cdkl5
- CDKL5_HUMAN
- Cyclin dependent kinase 5 transcript
see all -
Function
Mediates phosphorylation of MECP2. -
Tissue specificity
Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis. -
Involvement in disease
Note=Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X;6)(p22.3;q14); translocation t(X;7)(p22.3;p15).
Defects in CDKL5 are a cause of epileptic encephalopathy early infantile type 2 (EIEE2) [MIM:300672]; also known as atypical CDKL5-related Rett syndrome. EIEE2 is a severe form of epilepsy characterized by seizures or spasms beginning in infancy. Patients manifest features resembling Rett syndrome such as microcephaly, lack of speech development, stereotypic hand movements. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Nucleus. - Information by UniProt
Images
-
SDS-PAGE - Recombinant Human CDKL5 protein (ab131695)The purity of ab131695 was determined to be >90% by densitometry.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab131695 has not yet been referenced specifically in any publications.
