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    recombinant-human-cenpa-protein-tagged-ab275915.pdf

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Epigenetics and Nuclear Signaling Histones Variants
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Recombinant Human CENPA protein (Tagged) (ab275915)

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SDS-PAGE - Recombinant Human CENPA protein (Tagged) (ab275915)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Recombinant Human CENPA protein (Tagged)
      See all CENPA proteins and peptides
    • Expression system

      Wheat germ
    • Accession

      P49450-2
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGPRRRSRKP EAPRRRSPSP TPTPGPSRRG PSLGASSHQH SRRRQGWLKE IRKLQKSTHL LIRKLPFSRL AAEAFLVHLF EDAYLLTLHA GRVTLFPKDV QLARRIRGLE EGLG
      • Predicted molecular weight

        16 kDa
      • Amino acids

        1 to 114
      • Tags

        GST tag N-Terminus

    Associated products

    • Related Products

      • Anti-GST antibody [EPR4236] (ab111947)
      • Anti-CENPA antibody (ab217622)
      • Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
      • Anti-GST antibody (ab9085)

    Specifications

    Our Abpromise guarantee covers the use of ab275915 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 8.0
      Constituents: 0.79% Tris HCl, 0.31% Glutathione

    General Info

    • Alternative names

      • CENP A
      • CENP-A
      • cenpa
      • CENPA_HUMAN
      • Centromere autoantigen A
      • Centromere protein A
      • Centromere protein A 17kDa
      • Histone H3 like centromeric protein A
      • Histone H3-like centromeric protein A
      see all
    • Function

      Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
    • Sequence similarities

      Belongs to the histone H3 family.
    • Domain

      The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.
    • Post-translational
      modifications

      Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
      Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.
    • Cellular localization

      Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height.
    • Target information above from: UniProt accession P49450 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human CENPA protein (Tagged) (ab275915)
      SDS-PAGE - Recombinant Human CENPA protein (Tagged) (ab275915)

      12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab275915? Please let us know so that we can cite the reference in this datasheet.

    ab275915 has not yet been referenced specifically in any publications.

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